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Chemistry of Natural Compounds

, Volume 54, Issue 2, pp 330–333 | Cite as

Isolation, Characterization, and Antimicrobial Activity of Extracellular Polypeptides from Diazotrophic Strain Rhizobium radiobacter

  • P. Rozi
  • B. A. Rasulov
  • A. Waili
  • Y. Mijiti
  • Y. H. Gao
  • A. Yili
  • H. A. Aisa
Article

Antimicrobial polypeptide fractions were isolated from Rhizobium radiobacter culture medium and purified. Mass-spectrometric analysis showed that the most active biocidal peptide fraction contained 24 components with molecular masses in the range 1171.14–3626.78 Da. The isolated peptide fractions contained 60 and 56% protein, respectively, and inhibited the growth of test pathogens Candida albicans (CA) and Escherichia coli (EC). These polypeptides demonstrated protective properties.

Keywords

Rhizobium radiobacter isolation of extracellular polypeptides antimicrobial activity electrophoresis in PAAG chromatography-mass spectrometric analysis Candida albicans Escherichia coli 

Notes

Acknowledgment

The research was financially supported by the Chinese Program for National Scientific Research (No. U1403201), Key Technology and Application Demonstration of the Production and Value-added Processing of Chickpea (No. 2015 BAD29B03-4). We thank Central Asia Drug Research and Development Center of the Chinese Academy of Sciences, Key Laboratory of Plant Resources and Chemistry of Arid Zone, Xinjiang Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Beijing and the Academy of Sciences of the Republic of Uzbekistan for support of the research.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  • P. Rozi
    • 1
    • 3
  • B. A. Rasulov
    • 4
  • A. Waili
    • 1
    • 3
  • Y. Mijiti
    • 1
    • 3
  • Y. H. Gao
    • 1
    • 3
  • A. Yili
    • 1
    • 2
  • H. A. Aisa
    • 1
    • 2
  1. 1.Key Laboratory of Plants Resources and Chemistry of Arid Zone, Xinjiang Technical Institute of Physics and ChemistryChinese Academy of SciencesUrumqiP. R. China
  2. 2.State Key Laboratory Basis of Xinjiang Indigenous Medicinal Plants Resource Utilization, Xinjiang Technical Institute of Physics and ChemistryChinese Academy of SciencesUrumqiP. R. China
  3. 3.University of Chinese Academy of ScienceBeijingP. R. China
  4. 4.Institute of Plant Genetics and Experimental BiologyAcademy of Sciences of the Republic of UzbekistanKibraiskii DistrictUzbekistan

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