Isolation, Characterization, and Antimicrobial Activity of Extracellular Polypeptides from Diazotrophic Strain Rhizobium radiobacter
Antimicrobial polypeptide fractions were isolated from Rhizobium radiobacter culture medium and purified. Mass-spectrometric analysis showed that the most active biocidal peptide fraction contained 24 components with molecular masses in the range 1171.14–3626.78 Da. The isolated peptide fractions contained 60 and 56% protein, respectively, and inhibited the growth of test pathogens Candida albicans (CA) and Escherichia coli (EC). These polypeptides demonstrated protective properties.
KeywordsRhizobium radiobacter isolation of extracellular polypeptides antimicrobial activity electrophoresis in PAAG chromatography-mass spectrometric analysis Candida albicans Escherichia coli
The research was financially supported by the Chinese Program for National Scientific Research (No. U1403201), Key Technology and Application Demonstration of the Production and Value-added Processing of Chickpea (No. 2015 BAD29B03-4). We thank Central Asia Drug Research and Development Center of the Chinese Academy of Sciences, Key Laboratory of Plant Resources and Chemistry of Arid Zone, Xinjiang Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Beijing and the Academy of Sciences of the Republic of Uzbekistan for support of the research.
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