Structural Elucidation of Alkaloids from Aerial Parts of Rivea hypocrateriformis and Their Antihemolytic and Cytotoxic Activity
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Two new alkaloids, designated as hypocretine 1 and 2, have been extracted from the aerial parts of Rivea hypocrateriformis, and their structures were established as 1-(4-(((1R,5S,6R)-(4-acetyl-2,6-dibutylphenoxy)-8-methyl-8-azabicyclo[3.2.1]octan-6-yl)oxy)-2,6-dibutylphenyl)ethan-1-one and 1-(4-((3-(4-acetyl-2-butyl-6-(4-hydroxybutyl)phenoxy)-8-methyl-8-azabicyclo[3.2.1]octan-6-yl)oxy)-2-butyl-6-(4-hydroxybutyl)phenyl)ethan-1-one. These alkaloids were further characterized by mass and NMR spectral data analysis. Further studies were conducted to evaluate their antihemolytic and cytotoxic activity. The extracted compounds exhibited potent antihemolytic activity against cow erythrocytes. The results of the MTT assay for cytotoxicity revealed the antiproliferative activity of compounds 1and 2 against MCF-7 breast cancer cell line.
KeywordsRivea hypocrateriformis alkaloids hypocretine compounds antihemolytic activity cytotoxicity
The authors are grateful to the Chairman, Department of Chemistry, Gulbarga University, Gulbarga for providing the facility to carry out the research work. Also, we thank the University Grants Commission (F.No. 40-58/2011 (SR)) and F.No. 25-1/2013-14 (BSR)/F.No. 7-226/2009 (BSR)) for financial support in the form of the major research project and authorities of the Department of Pharmacology, Manipal College of Pharmaceutical Sciences, Manipal University, Manipal for providing the testing facilities. Dr. S. Yallappa, thankful to the principal, Gokhale Centenary College, Ankola and gratefully acknowledge UGC, New Delhi for College with Potential for Excellence Grant (F.No. 1044/2016, N.S/P.E).
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