Homogeneous alginate lyase with rare specificity exhibiting maximum activity at pH 5.0 and temperature range 40–55°C that catalyzed cleavage of 1→4 glycoside bonds between α-L-guluronic acid residues was isolated from liver-pancreas of the marine gastropod mollusk Lambis sp. The alginate lyase was classified as a poly-1→4-α-L-guluronate lyase (EC 4.2.2.11).
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T. Y. Wong, L. A. Preston, and N. L. Schiller, Annu. Rev. Microbiol., 54, 289 (2000).
P. Gacesa, FEBS Lett., 212, 199 (1987).
P. Michaud, A. Da Costa, B. Courtois, and J. Courtois, Crit. Rev. Biotechnol., 23, 233 (2003).
Y. Iwamoto, K. Iriyama, K. Osatomi, T. Oda, and T. Muramatsu, J. Protein Chem., 21, 455 (2002).
J. W. Li, S. Dong, J. Song, C. B. Li, X. L. Chen. B. B. Xie, and Y. Z. Zhang, Mar. Drugs, 9, 109 (2011).
C. Boyen, B. Kloareg, M. Poline-Fuller, and A. Gibor, Phycologia, 29, 173 (1990).
H. I. Nakada and P. C. Sweeny, J. Biol. Chem., 242, 845 (1967).
V. V. Favorov, E. I. Vozhova, V. A. Denisenko, and L. A. Elyakova, Biochim. Biophys. Acta, 569, 259 (1979).
T. N. Zvyagintseva, N. M. Shevchenko, E. L. Nazarenko, V. I. Gorbach, A. M. Urvantseva, M. I. Kiseleva, and V. V. Isakov, J. Exp. Mar. Biol. Ecol., 320, 123 (2005).
M. M. Bradford, Anal. Biochem., 72, 248 (1976).
M. Dubois, K. A. Gilles, J. Hamilton, P. A. Robers, and F. Smith, Anal. Chem., 18, 350 (1956).
T. E. Nelson, J. Biol. Chem., 153, 375 (1944).
U. K. Laemmli, Nature, 227, 680 (1970).
Acknowledgment
The work was supported by the Russian Foundation for Basic Research (No. 12-04-31183, 12-04-00669-a) and the RAS Program “Molecular and Cellular Biology.”
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Translated from Khimiya Prirodnykh Soedinenii, No. 2, March–April, 2013, pp. 188–190.
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Sil′chenko, A.S., Kusaikin, M.I., Zakharenko, A.M. et al. Isolation from the marine mollusk Lambis sp. and catalytic properties of an alginate lyase with rare substrate specificity. Chem Nat Compd 49, 215–218 (2013). https://doi.org/10.1007/s10600-013-0564-6
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DOI: https://doi.org/10.1007/s10600-013-0564-6