Polysaccharides of Fabaceae. I. Galactomannan of Astragalus sericeocanus seeds
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Galactomannan (yield 3.58% of seed mass) of molecular weight 876 kDa was isolated from seeds of Astragalus sericeocanus Gontsch. (Fabaceae). Its solutions had high viscosity [η], 764.6 mL/g, and optical density [α]D +65.3°. The polysaccharide consisted of galactose and mannose in molar ratio 1:1.58. The main chain of the galactomannan macromolecule was constructed of 1,4-β-D-mannopyranose units, 63% of which were substituted at C-6 by single α-D-galactopyranose units. 13C NMR spectroscopy established that the galactomannan contained units of differently substituted galactose mannobiose units: Man-Man, (Gal)Man-Man, and/or Man-Man(Gal) in addition to (Gal)Man-Man(Gal), the ratio of which was 0.15:0.51:0.34.
Key wordsAstragalus sericeocanus Fabaceae galactomannan 13C NMR spectroscopy.
We thank Senior Scientist of the Institute of General and Experimental Biology (Ulan-Ude), Candidate of Biological Sciences D. V. Sandanov for supplying samples of A. sericeocanus seeds.
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