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Chitin-binding antifungal protein from Ficus carica latex

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Abstract

A low-molecular-weight protein with antifungal activity was isolated from freshly collected latex of the Inzhir tree (Ficus carica L.) by successive affinity chromatography over chitin, cation-exchange chromatography over SP-Sephadex C-50, and reversed-phase HPLC. The molecular weight of 6481 and the partial N-terminus sequence of the protein were determined (MALDI-TOFMS).

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References

  1. W. F. Broekaert, B. P. Cammue, M. F. De Bolle, K. Thevissen, G. W. de Samblanx, and R. W. Osborn, Crit. Rev. Plant Sci., 16, 297 (1997).

    Article  CAS  Google Scholar 

  2. F. Garcia-Olmedo, A. Molina, J. M. Alamillo, and P. Rodrigues-Palenzuela, Biopolymers, 479 (1998).

  3. H. K. No, P. Meyers, and K. S. Lee, J. Agric. Food Chem., 37, 575 (1989).

    Article  CAS  Google Scholar 

  4. T. Iwai, H. Kaku, R. Honkura, S. Nakamura, H. Ochiai, T. Sasaki, and Y. Ohashi, Mol. Plant-Microbe Interact., 15, 6, 515 (2002).

    Article  Google Scholar 

  5. D. Kerk, J. Bulgrein, D. W. Smith, and M. Gribskov, Plant Physiol., 131, 1209 (2003).

    Article  PubMed  CAS  Google Scholar 

  6. C. P. Selitrennikoff, Appl. Environ. Microbiol., 67, 2883 (2001).

    Article  PubMed  CAS  Google Scholar 

  7. R. J. Simpson, Protein and Proteomic, CSHL Press, (2003), p. 83.

  8. F. Gonzalez, G. Cardenas, and N. Marmolejo, Anal. Biochem., 197, No. 1, 276 (1991).

    Article  Google Scholar 

  9. J. D. Thompson, T. J. Gibson, F. Plewniak, F. Jeanmougin, and D. G. Higgins, Nucleic Acid Res., 25, 4876 (1997).

    Article  PubMed  CAS  Google Scholar 

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ACKNOWLEDGMENT

We thank I. V. Nazimov (Institute of Bioorganic Chemistry, RAS, Moscow) for determining the partial N-terminus sequence of the antifungal protein and for obtaining its mass spectrum and N. N. Kuznetsova (Institute of Bioorganic Chemistry, AS RU, Tashkent) for determining the protein toxicity.

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Authors and Affiliations

  1. Institute of Genetics and Experimental Biology, Academy of Sciences of the Republic of Uzbekistan, 102151, Tashkent Oblast’, Yukori-Yuz, Uzbekistan

    G. T. Mavlonov, Kh. A. Ubaidullaeva, M. I. Rakhmanov, I. Yu. Abdurakhmonov & A. Abdukarimov

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  1. G. T. Mavlonov
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  2. Kh. A. Ubaidullaeva
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  3. M. I. Rakhmanov
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  4. I. Yu. Abdurakhmonov
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  5. A. Abdukarimov
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Translated from Khimiya Prirodnykh Soedinenii, No. 2, pp. 171–173, March–April, 2008.

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Mavlonov, G.T., Ubaidullaeva, K.A., Rakhmanov, M.I. et al. Chitin-binding antifungal protein from Ficus carica latex. Chem Nat Compd 44, 216–219 (2008). https://doi.org/10.1007/s10600-008-9018-y

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  • DOI: https://doi.org/10.1007/s10600-008-9018-y

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