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Isolation and purification of lignoperoxidase from the mushroom Pleurotus ostreatus

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Abstract

The accumulation dynamics of lignolytic enzymes in culture media of the basidiomycetes Panus tigrinus, Pleurotus ostreatus, Fomes fomentarus, and the micromycete Aspergillus terreus were studied during the incubation period. It was found that Pleurotus ostreatus is the most active producer of lignoperoxidase enzymes among the studied fungi. Gel filtration and ion-exchange chromatography were used to isolate a homogeneous enzyme with lignoperoxidase activity. The maximum activity was found at pH 2.7 and 29°C. Gel electrophoresis determined the molecular weight (44 kDa).

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Authors and Affiliations

  1. Institute of Bioorganic Chemistry, Uzbekistan, Tashkent

    Sh. Ya. Mirzaakhmedov, Zh. F. Ziyavitdinov, A. B. Saliev & D. T. Ruzmetova

  2. Institute of Microbiology, Uzbekistan, 700128, Tashkent, ul. A. Kadyri, 7b

    Z. R. Akhmedova

  3. Institute of Electronics, Uzbekistan, 100125, Tashkent, ul. F. Khodzhaeva, 33

    S. T. Azizov

  4. University of Milan, DISTAM, via Celoria 2, 20133, Milano, Italy

    Dimitrios Fessas & Stefania Iametti

Authors
  1. Sh. Ya. Mirzaakhmedov
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  2. Zh. F. Ziyavitdinov
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  3. Z. R. Akhmedova
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  4. A. B. Saliev
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  5. D. T. Ruzmetova
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  6. S. T. Azizov
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  7. Dimitrios Fessas
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  8. Stefania Iametti
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Corresponding author

Correspondence to Sh. Ya. Mirzaakhmedov.

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Translated from Khimiya Prirodnykh Soedinenii, No. 6, pp. 564–566, November–December, 2007.

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Mirzaakhmedov, S.Y., Ziyavitdinov, Z.F., Akhmedova, Z.R. et al. Isolation and purification of lignoperoxidase from the mushroom Pleurotus ostreatus . Chem Nat Compd 43, 682–684 (2007). https://doi.org/10.1007/s10600-007-0227-6

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  • DOI: https://doi.org/10.1007/s10600-007-0227-6

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