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Isolation and Identification of Peptides from Soy 11S-Globulin with Hypocholesterolemic Activity


A peptide with hypocholesterolemic activity was isolated by HPLC from the pepsin hydrolysate of 11S-globulin. Its molecular weight (755.2 Da) and amino-acid sequence (Ile-Ala-Val-Pro-Gly-Glu-Val-Ala) were established. The hypocholesterolemic effect was determined by analysis of bile-acid binding and the percent inhibition of 3-hydroxy-3-methylglutaryl coenzyme A reductase in vitro. The lowering of the cholesterol content is explained by bile acids bound to hydrolysate peptides shielding them from reabsorption and stimulating the transformation of cholesterol in blood plasma.

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Translated from Khimiya Prirodnykh Soedinenii, No. 6, pp. 585–588, November–December, 2005.

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Pak, V.V., Koo, M.S., Kasymova, T.D. et al. Isolation and Identification of Peptides from Soy 11S-Globulin with Hypocholesterolemic Activity. Chem Nat Compd 41, 710–714 (2005).

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Key words

  • soy 11S- and 7S-globulins
  • pepsin hydrolysate
  • hypocholesterolemic effect