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Melibiase in Immobilized Cells of Watermelon

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Abstract

Cells of suspension culture Citrullus vulgaris cv. “Samara” were permeabilized by Tween 80 and immobilized by glutaraldehyde. The highest melibiase activity was at pH 5.4 and 60°C. The hydrolysis of substrate was linear for 3.5 h, reaching 65–70% conversion of the substrate. The cells, characterized by high enzyme activity and stability in long-term storage, showed convenient physico-mechanical properties (physical protection from shear forces and easy separation of product from biocatalysts).

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Authors and Affiliations

  1. Garden of Medicinal Plants, Faculty of Pharmacy, Comenius University, Odbojarov 10, 83232, Bratislava 3, Slovak Republic

    J. Stano & V. Foltan

  2. Department of Biophysics, National University of Uzbekistan, Vuzgorodok, 700174, Tashkent, Uzbekistan Republic

    E. Tokhtaeva

  3. Department of Botany, Faculty of Natural Sciences, Comenius University, Rehakova 39, 81101, Bratislava 1, Slovak Republic

    K. Micieta

  4. Department of Organization and Management in Pharmacy, Faculty of Pharmacy, Comenius University, Odbojarov 10, 83232, Bratislava 3, Slovak Republic

    M. Fulmekova

  5. Faculty of Pharmacy, Comenius University, Odbojarov 10, 83232, Bratislava 3, Slovak Republic

    M. Varadinova & M. Duricek

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  1. J. Stano
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  2. E. Tokhtaeva
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  5. M. Varadinova
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__________

Published in Khimiya Prirodnykh Soedinenii, No. 1, pp. 53–55, January–February, 2005.

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Stano, J., Tokhtaeva, E., Micieta, K. et al. Melibiase in Immobilized Cells of Watermelon. Chem Nat Compd 41, 65–68 (2005). https://doi.org/10.1007/s10600-005-0076-0

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  • DOI: https://doi.org/10.1007/s10600-005-0076-0

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