Abstract
The restricted range, taxonomic uncertainties and the apparent phylopatric behavior of Parotocinclus (dwarf plecos) make this fish group an excellent model to infer diversification patterns in Neotropical rivers. Thus, the aim of this study was to investigate the spatial–temporal relationships and the presence of cryptic forms in Parotocinclus cristatus from poorly known and human-impacted drainages in northeastern Brazil based on mitochondrial COI (589 bp) and Cyt-b (835 bp) and nuclear (Rhodopsin, 473 bp) markers. The phylogenetic reconstruction, haplotype network and species delimitation algorithms revealed three conspicuous and restricted molecular operational units (MOTUs) within P. cristatus. The samples from Cachoeira and Una basins represent a single MOTU dating back to the Early Pliocene, resulting in deep genetic divergence when compared to the other lineages. The MOTUs from Contas and Almada river basins diverged around 2.1 Ma, while the latter encompassed highly structured populations between upper and lower watersheds (0.6 Ma, Pleistocene). The closest relationship between more distant basins than nearby drainages indicates an intricate scenario of headwater captures that challenge traditional studies based on current geographic reliefs and ichthyofauna inventories. Therefore, caution when biogeographic inferences are carried out without further understanding about the genetic diversity and the geomorphological history, as commonly reported in fishes from eastern basins in Brazil. Finally, the conspicuous Linnean and Wallacean shortfalls in dwarf plecos, including unique lineages from unprotected and highly degraded areas, justifies a thorough review of their taxonomic status since valid species are the major source of information for defining conservation policies.
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Availability of data and material
The genetic data are available in BOLD Systems (project “Phylogeography of Parotocinclus cristatus—PCA” as part of the FISHBOL campaign) and Supplementary Material.
References
Anjos MS, Bitencourt JA, Nunes LA et al (2020) Species delimitation based on integrative approach suggests reallocation of genus in Hypostomini catfish (Siluriformes, Loricariidae). Hydrobiologia 847:563–578. https://doi.org/10.1007/s10750-019-04121-z
Armbruster JW (2004) Phylogenetic relationships of the suckermouth armoured catfishes (Loricariidae) with emphasis on the Hypostominae and the Ancistrinae. Zool J Linn Soc 141:1–80. https://doi.org/10.1111/j.1096-3642.2004.00109.x
Azevedo-Santos VM, Frederico RG, Fagundes CK et al (2018) Protected areas: a focus on Brazilian freshwater biodiversity. Divers Distrib 25:442–448. https://doi.org/10.1111/ddi.12871
Bandelt HJ, Forster P, Rohl A (1999) Median-joining networks for inferring intraspecific phylogenies. Mol Biol Evol 16:37–48. https://doi.org/10.1093/oxfordjournals.molbev.a026036
Barreto SB, Silva AT, Batalha-Filho H, Affonso PRAM, Zanata AM (2018) Integrative approach reveals a new species of Nematocharax (Teleostei: Characidae). J Fish Biol. https://doi.org/10.1111/jfb.13834
Behrens-Chapuis S, Herder F, Esmaeili HR et al (2015) Adding nuclear rhodopsin data where mitochondrial COI indicates discrepancies—can this marker help to explain conflicts in cyprinids? DNA Barcodes 3:187–199. https://doi.org/10.1515/dna-2015-0020
Bini LM, Diniz-Filho JA, Rangel TFLVB et al (2006) Challenging Wallacean and Linnean shortfalls: knowledge gradients and conservation planning in a biodiversity hotspot. Divers Distrib 12:475–482. https://doi.org/10.1111/j.1366-9516.2006.00286.x
Bishop P (1995) Drainage rearrangement by river capture, beheading and diversion. Prog Phys Geogr 19:449–473. https://doi.org/10.1177/030913339501900402
Bitencourt JA, Affonso PRAM, Giuliano-Caetano L et al (2011) Identification of distinct evolutionary units in allopatric populations of Hypostomus cf. wuchereri Günther, 1864 (Siluriformes: Loricariidae): karyotypic evidence. Neotrop Ichthyol 9:317–324. https://doi.org/10.1590/s1679-62252011000200008
Bizerril CRSF (1994) Análise taxonômica e biogeográfica da ictiofauna de água doce do leste brasileiro. Acta Biol Leopold 16:51–80
Blair C, Bryson RW Jr (2017) Cryptic diversity and discordance in single-locus species delimitation methods within horned lizards (Phrynosomatidae: Phrynosoma). Mol Ecol Resour 17:1168–1182. https://doi.org/10.1111/1755-0998.12658
Blessing JJ, Marshall JC, Balcombe SR (2010) Humane killing of fishes for scientific research: a comparison of two methods. J Fish Biol 76:2571–2577. https://doi.org/10.1111/j.1095-8649.2010.02633.x
Britski HA, Garavello JC (2009) Redescription of Parotocinclus bahiensis (Miranda-Ribeiro, 1918) (Pisces, Ostariophysi, Loricariidae). Zootaxa 2143:59–67
Camelier P, Zanata AM (2014) Biogeography of freshwater fishes from the Northeastern Mata Atlântica freshwater ecoregion: distribution, endemism, and area relationships. Neotrop Ichthyol 12:683–698. https://doi.org/10.1590/1982-0224-20130228
Camelier P, Menezes NA, Costa-Silva GJ et al (2018) Molecular and morphological data of the freshwater fish Glandulocauda melanopleura (Characiformes: Characidae) provide evidences of river captures and local differentiation in the Brazilian Atlantic Forest. PLoS ONE 13:e0194247. https://doi.org/10.1371/journal.pone.0194247
Casciotta J, Almirón A, Sánchez S et al (2013) The genus Gymnotus (Gymnotiformes: Gymnotidae) in Argentina. How bad taxonomy results in poor regulations and no conservation. J Appl Ichthyol 29:208–212. https://doi.org/10.1111/jai.12028
Castro RMC (1999) Evolução da ictiofauna de riachos sul-americanos: padrões gerais e possíveis processos causais. In: Caramaschi EP, Mazzoni R, Peres-Neto PR (eds) Ecologia de Peixes de Riachos, 6rd edn. Rio de Janeiro, RJ, pp 1–22
Eschmeyer WN, Fricke R, Van Der Laan R (eds) (2019) Catalog of fishes: Loricariidae. Publishing Eschmeyer's Catalog of Fishes. http://researcharchive.calacademy.org/research/ichthyology/catalog/fishcatmain.asp. Accessed 21 July 2019
Cetra M, Ferreira FC, Carmassi AL (2009) Caracterização das assembleias de peixes de riachos de cabeceira no período chuvoso na bacia do rio Cachoeira (SE da Bahia, NE do Brasil). Biota Neotrop 29:107–115. https://doi.org/10.1590/S1676-06032009000200010
Cetra M, Sarmento-Soares LM, Martins-Pinheiro RF (2010) Peixes de riachos e novas Unidades de Conservação no sul da Bahia. Pan-Am J Aquat Sci 5:11–21
Christofoletti A (1975) Capturas fluviais. In: Enciclopédia Mirador Internacional, 5rd edn, São Paulo, SP, pp 2.049–2.05
Corander J, Marttinen P, Sirén J et al (2008) Enhanced Bayesian modelling in BAPS software for learning genetic structures of populations. BMC Bioinform 9:539. https://doi.org/10.1186/1471-2105-9-539
Cramer CA, Bonatto SL, Reis RE (2011) Molecular phylogeny of the Neoplecostominae and Hypoptopomatinae (Siluriformes: Loricariidae) using multiple genes. Mol Phylogenet Evol 59:43–52. https://doi.org/10.1016/j.ympev.2011.01.002
Darriba D, Taboada GL, Doallo R et al (2012) JModelTest 2: more models, new heuristics and parallel computing. Nat Methods 9:772–772. https://doi.org/10.1038/nmeth.2109
DePaula FCF, Silva DML, Souza CM (2012) Tipologias Hidroquímicas das Bacias Hidrográficas do Leste da Bahia. Rev Virtual Quim 4:365–373
Dias MS, Oberdorff T, Hugueny B et al (2014) Global imprint of historical connectivity on freshwater fish biodiversity. Ecol Lett 17:1130–1140. https://doi.org/10.1111/ele.12319
Dupanloup I, Schneider S, Excoffier L (2002) A simulated annealing approach to define the genetic structure of populations. Mol Ecol 11:2571–2581. https://doi.org/10.1046/j.1365-294x.2002.01650.x
Excoffier L, Lischer HE (2010) Arlequin suite ver 3.5: a new series of programs to perform population genetics analyses under Linux and Windows. Mol Ecol Resour 10:564–567. https://doi.org/10.1111/j.1755-0998.2010.02847.x
Excoffier L, Smouse PE, Quattro JM (1992) Analysis of molecular variance inferred from metric distances among DNA haplotypes: application to human mitochondrial DNA restriction data. Genetics 131:479–491
Floyd R, Abebe E, Papert A et al (2002) Molecular barcodes for soil nematode identification. Mol Ecol 11:839–850. https://doi.org/10.1046/j.1365-294x.2002.01485.x
Fu YX (1997) Statistical tests of neutrality of mutations against population growth, hitchhiking and background selection. Genetics 147:915–925
Fujisawa T, Barraclough TG (2013) Delimiting species using single-locus data and the Generalized Mixed Yule Coalescent approach: a revised method and evaluation on simulated data sets. Syst Biol 62:707–724. https://doi.org/10.1093/sysbio/syt033
Garavello JC (1977) Systematics and geographical distribution of the genus Parotocinclus Eigenmann & Eigenmann, 1889 (Ostariophysi, Loricariidae). Arq Zool 28:1–37
Garavello JC (1988) Three new species of Parotocinclus Eigenmann & Eigenmann, 1889 with comments on their geographical distribution (Pisces, Loricariidae). Naturalia 13:117–128
Gauger MF, Buckup PA (2005) Two new species of Hypoptopomatinae from the rio Paraíba do Sul basin, with comments on the monophyly of Parotocinclus and the Otothyrini (Siluriformes: Loricariidae). Neotrop Ichthyol 3:509–518. https://doi.org/10.1590/s1679-62252005000400008
Gery J (1969) The fresh-water fishes of South America. Monogr Biol 19:828–848
Gomes RL, Moraes ME, Moreau AM et al (2012) Aspectos físico-ambientais e de uso e ocupação do solo da bacia hidrográfica do rio Almada-BA. Bol Geogr 30:45–57. https://doi.org/10.4025/bolgeogr.v30i2.16423
Hall TA (1999) BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp Ser 41:95–98
Hebert PD (2004) Ten species in one: DNA Barcoding reveals cryptic species in the neotropical skipper butterfly Astraptes fulgerator. Proc Natl Acad Sci USA 101(41):14812
Hebert PDN, Cywinska A, Ball SL et al (2003) Biological identifications through DNA barcodes. Proc R Soc Lond [Biol] 270:313–321
Heled J, Drummond AJ (2008) Bayesian inference of population size history from multiple loci. BMC Evol Biol 8:289. https://doi.org/10.1186/1471-2148-8-289
Hortal J, De Bello F, Diniz-Filho JA et al (2015) Seven shortfalls that beset large-scale knowledge of biodiversity. Annu Rev Ecol Evol Syst 46:523–549. https://doi.org/10.1146/annurev-ecolsys-112414-054400
Huelsenbeck JP, Ronquist F (2001) MRBAYES: Bayesian inference of phylogenetic trees. Bioinformatics 17:754–755
Ivanova NV, Zemlak TS, Hanner RH et al (2007) Universal primer cocktails for fish DNA barcoding. Mol Ecol Notes 7:544–548. https://doi.org/10.1111/j.1471-8286.2007.01748.x
Jensen J, Bohonak A, Kelley S (2005) Isolation by distance, web service. BMC Genet 6:13. https://doi.org/10.1186/1471-2156-6-13
Kapli P, Lutteropp S, Zhang J et al (2017) Multi-rate poisson tree processes for single-locus species delimitation under maximum likelihood and Markov chain Monte Carlo. Bioinformatics 33:1630–1638. https://doi.org/10.1093/bioinformatics/btx025
Kimura M (1980) A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16:111–120. https://doi.org/10.1007/bf01731581
Kumar S, Stecher G, Li M et al (2018) MEGA X: molecular evolutionary genetics analysis across computing platforms. Mol Biol Evol 35:1547–1549. https://doi.org/10.1093/molbev/msy096
Lehmann AP, Lima FCT, Reis RE (2018) Parotocinclus yaka, a new species of armored catfish (Loricariidae: Hypoptopomatinae), from the Amazon basin in Brazil. Zootaxa 4521:584. https://doi.org/10.11646/zootaxa.4521.4.7
Leonardo R, Nunes RS, Monteiro ML (2016) Molecular testing on sardines and rulings on the authenticity and nutritional value of marketed fishes: an experience report in the state of Rio de Janeiro, Brazil. Food Control 60:394–400. https://doi.org/10.1016/j.foodcont.2015.08.004
Lundberg JG, Marshall LG, Guerrero J et al (1998) The stage for neotropical fish diversification: a history of tropical South American rivers. In: Malabarba LR, Reis RE, Vari RP et al (eds) Phylogeny and classification of neotropical fishes. EDUPUCRS, Porto Alegre, pp 13–48
Mantel N (1967) The detection of disease clustering and a generalized regression approach. Cancer Res 27:209–220
Medrado AS, Ribeiro MS, Affonso PRAM et al (2012) Cytogenetic divergence in two sympatric fish species of the genus Astyanax Baird and Girard, 1854 (Characiformes, Characidae) from northeastern Brazil. Genet Mol Biol 35:797–801. https://doi.org/10.1590/s1415-47572012005000075
Medrado AS, Affonso PRAM, Carneiro PL et al (2015) Allopatric divergence in Astyanax aff. fasciatus Cuvier, 1819 (Characidae, Incertae sedis) inferred from DNA mapping and chromosomes. Zool Anz 257:119–129. https://doi.org/10.1016/j.jcz.2015.05.005
Menezes NA, Weitzman SH, Oyakawa OT et al (2007) Peixes de água doce da Mata Atlântica: Lista preliminar das espécies e comentários sobre conservação de peixes de água doce neotropicais. Museu de Zoologia da Universidade de São Paulo, São Paulo
Miller MA, Pfeiffer W, Schwartz T (2010) Creating the CIPRES Science Gateway for inference of large phylogenetic trees. 2010 Gateway Computing Environments Workshop (GCE). https://doi.org/10.1109/gce.2010.5676129
Montoya-Burgos JI (2003) Historical biogeography of the catfish genus Hypostomus (Siluriformes: Loricariidae), with implications on the diversification of Neotropical ichthyofauna. Mol Ecol 12:1855–1867. https://doi.org/10.1046/j.1365-294x.2003.01857.x
Nacif PGS (2000) Ambientes naturais da bacia hidrográfica do rio Cachoeira, com ênfase nos domínios pedológicos. PhD Thesis, Universidade Federal de Viçosa, Viçosa, Minas Gerais, Brazil
Niemiller ML, Graening GO, Fenolio DB et al (2013) Doomed before they are described? The need for conservation assessments of cryptic species complexes using an amblyopsid cavefish (Amblyopsidae: Typhlichthys) as a case study. Biodivers Conserv 22:1799. https://doi.org/10.1007/s10531-013-0514-4
Nogueira C, Buckup PA, Menezes NA et al (2010) Restricted-range fishes and the conservation of Brazilian freshwaters. PLoS ONE 5:e11390. https://doi.org/10.1371/journal.pone.0011390
Oliveira IA, Argolo LA, Bitencourt JD et al (2016) Cryptic chromosomal diversity in the complex “Geophagus” brasiliensis (Perciformes, Cichlidae). Zebrafish 13:33–44. https://doi.org/10.1089/zeb.2015.1169
Ornelas-García C, Domínguez-Domínguez O, Doadrio I (2008) Evolutionary history of the fish genus Astyanax Baird & Girard (1854) (Actinopterygii, Characidae) in Mesoamerica reveals multiple morphological homoplasies. BMC Evol Biol 8:340. https://doi.org/10.1186/1471-2148-8-340
Page RD (2016) DNA barcoding and taxonomy: dark taxa and dark texts. Philos Trans R Soc B 371:20150334. https://doi.org/10.1098/rstb.2015.0334
Paithankar KR, Prasad KS (1991) Precipitation of DNA by polyethylene glycol and ethanol. Nucleic Acids Res 19:1346. https://doi.org/10.1093/nar/19.6.1346
Perdices A, Bermingham E, Montilla A et al (2002) Evolutionary history of the genus Rhamdia (Teleostei: Pimelodidae) in Central America. Mol Phylogenet Evol 25:172–189. https://doi.org/10.1016/s1055-7903(02)00224-5
Pereira LHG, Hanner R, Foresti F, Oliveira C (2013) Can DNA barcoding accurately discriminate megadiverse Neotropical freshwater fish fauna? BMC Genet. https://doi.org/10.1186/1471-2156-14-20
Puillandre N, Lambert A, Brouillet S et al (2012) ABGD, Automatic Barcode Gap Discovery for primary species delimitation. Mol Ecol 21:1864–1877. https://doi.org/10.1111/j.1365-294x.2011.05239.x
Rambaut A, Drummond AJ, Xie D et al (2018) Posterior summarization in Bayesian phylogenetics using Tracer 1.7. Syst Biol 67:901–904. https://doi.org/10.1093/sysbio/syy032
Ramos TP, Lehmann AP, Barros-Neto LF et al (2016) Redescription of the endangered hypoptopomatine catfish Parotocinclus spilurus (Fowler, 1941) (Siluriformes: Loricariidae) from the upper rio Jaguaribe basin, northeastern Brazil. Neotrop Ichthyol 14:e150098. https://doi.org/10.1590/1982-0224-20150098
Ramos-Onsins SE, Rozas J (2002) Statistical properties of new neutrality tests against population growth. Mol Biol Evol 19:2092–2100. https://doi.org/10.1093/oxfordjournals.molbev.a004034
Ratnasingham S, Hebert PD (2007) BOLD: the Barcode of life data system (http://www.barcodinglife.org). Mol Ecol Notes 7:355–364. https://doi.org/10.1111/j.1471-8286.2007.01678.x
Ratnasingham S, Hebert PD (2013) A DNA-based registry for all animal species: the barcode index number (BIN) system. PLoS ONE 8:e66213. https://doi.org/10.1371/journal.pone.0066213
Reis RE, Albert JS, Di Dario F et al (2016) Fish biodiversity and conservation in South America. J Fish Biol 89:12–47. https://doi.org/10.1111/jfb.13016
Ribeiro AC (2006) Tectonic history and the biogeography of the freshwater fishes from the coastal drainages of eastern Brazil: an example of faunal evolution associated with a divergent continental margin. Neotrop Ichthyol 4:225–246. https://doi.org/10.1590/s1679-62252006000200009
Ribeiro AC, Lima FCT, Menezes NA (2016) Biogeografia dos Peixes de Água Doce da América do Sul. In: Carvalho CJB, Almeida EAB (eds) Biogeografia da América do Sul: análise de tempo, espaço e forma. Roca, Rio de Janeiro, pp 245–258
Ribeiro AC, Riccomini C, Leite JAD (2018) Origin of the largest South American transcontinental water divide. Sci Rep 8:17144. https://doi.org/10.1038/s41598-018-35554-6
Rice WR (1989) Analyzing tables of statistical tests. Evolution 43:223–225. https://doi.org/10.1111/j.1558-5646.1989.tb04220.x
Roxo FF, Albert JS, Silva GSC et al (2014) Molecular phylogeny and biogeographic history of the armored neotropical Catfish Subfamilies Hypoptopomatinae, Neoplecostominae and Otothyrinae (Siluriformes: Loricariidae). PLoS ONE 9:e105564. https://doi.org/10.1371/journal.pone.0105564
Roxo FF, Silva GSC, Ochoa LE et al (2015) Description of a new genus and three new species of Otothyrinae (Siluriformes, Loricariidae). ZooKeys 534:103–134. https://doi.org/10.3897/zookeys.534.6169
Roxo FF, Lujan NK, Tagliacollo VA et al (2017b) Shift from slow- to fast-water habitats accelerates lineage and phenotype evolution in a clade of Neotropical suckermouth catfishes (Loricariidae: Hypoptopomatinae). PLoS ONE 12:e0178240. https://doi.org/10.1371/journal.pone.0178240
Roxo FF, Melo BF, Silva GSC et al (2017a) New species of Parotocinclus (Siluriformes: Loricariidae) from coastal drainages of Rio de Janeiro, southeastern Brazil. Zootaxa 4232:260. https://doi.org/10.11646/zootaxa.4232.2.9
Roxo FF, Ochoa LE, Sabaj MH et al (2019) Phylogenomic reappraisal of the Neotropical catfish family Loricariidae (Teleostei: Siluriformes) using ultraconserved elements. Mol Phylogenet Evol 135:148–165. https://doi.org/10.1016/j.ympev.2019.02.017
Rozas J, Ferrer-Mata A, Sánchez-DelBarrio JC et al (2017) DnaSP 6: DNA sequence polymorphism analysis of large data sets. Mol Biol Evol 34:3299–3302. https://doi.org/10.1093/molbev/msx248
Saadi A, Machette, MN, Haller, KM et al (2002) Map and database of quaternary faults and lineaments in Brazil. Publishing from U.S. Geological Survey Open-File Report 02-230, Version 1.0. http://pubs.usgs.gov/of/2002/ofr-02-230/. Accessed 20 Mar 2019
Saitou N, Nei M (1987) The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425. https://doi.org/10.1093/oxfordjournals.molbev.a040454
Sarmento-Soares LM, Lehmann AP, Martins-Pinheiro RF (2009) Parotocinclus arandai, a new species of hypoptopomatine catfish (Siluriformes: Loricariidae) from the upper rios Jucuruçu and Buranhém, States of Bahia and Minas Gerais, Brazil. Neotrop Ichthyol 7:191–198. https://doi.org/10.1590/s1679-62252009000200009
Schaefer SA (2003) Subfamily Hypoptopomatinae (Armored catfishes). In: Reis RE, Kullander SO, Ferraris CJ (eds) Check list of the freshwater fishes of South and Central America. EDIPUCRS, Porto Alegre, Rio Grande do Sul, pp 321–329
Severi W, El-deir ACA, Felix RTS et al (2010) Composição e abundância da ictiofauna na área de influencia dos reservatórios de Pedra e Funil, bacia do Rio de Contas, Bahia. In: Moura NA, Araújo EL, Bittencourt-Oliveira MC et al (eds) Reservatórios do Nordeste do Brasil: biodiversidade, ecologia e manejo. NUPEEA, Bauru, pp 541–572
Sevilla RG, Diez A, Norén M et al (2007) Primers and polymerase chain reaction conditions for DNA barcoding teleost fish based on the mitochondrial cytochrome b and nuclear rhodopsin genes. Mol Ecol Notes 7:730–734. https://doi.org/10.1111/j.1471-8286.2007.01863.x
Slatkin M (1987) Gene flow and the geographic structure of natural populations. Science 236:787–792. https://doi.org/10.1126/science.3576198
Souza CR, Affonso PRAM, Bitencourt JÁ et al (2018) Species validation and cryptic diversity in the Geophagus brasiliensis Quoy & Gaimard, 1824 complex (Teleostei, Cichlidae) from Brazilian coastal basins as revealed by DNA analyses. Hydrobiologia 809:309–321. https://doi.org/10.1007/s10750-017-3482-y
Stamatakis A (2014) RAxML version 8: a tool for phylogenetic analysis and post-analysis of large phylogenies. Bioinformatics 30:1312–1313. https://doi.org/10.1093/bioinformatics/btu033
Strecker U, Faúndez VH, Wilkens H (2004) Phylogeography of surface and cave Astyanax (Teleostei) from Central and North America based on cytochrome b sequence data. Mol Phylogenet Evol 33:469–481. https://doi.org/10.1016/j.ympev.2004.07.001
Suchard MA, Lemey P, Baele G et al (2018) Bayesian phylogenetic and phylodynamic data integration using BEAST 1.10. Virus Evol 4:vey016. https://doi.org/10.1093/ve/vey016
Tajima F, Misawa K, Innan H (1998) The amount and pattern of DNA polymorphism under the neutral mutation hypothesis. Genetics 102–103:103–107. https://doi.org/10.1007/978-94-011-5210-5_10
Tang C, Humphreys AM, Fontaneto D et al (2014) Effects of phylogenetic reconstruction method on the robustness of species delimitation using single-locus data. Methods Ecol Evol 5:1086–1094. https://doi.org/10.1111/2041-210X.122461
Thomaz AT, Knowles LL (2018) Flowing into the unknown: inferred paleodrainages for studying the ichthyofauna of Brazilian coastal rivers. Neotrop Ichthyol 16(16):e180019. https://doi.org/10.1590/1982-0224-20180019
Thomaz AT, Malabarba LR, Bonatto SL et al (2015) Testing the effect of palaeodrainages versus habitat stability on genetic divergence in riverine systems: study of a Neotropical fish of the Brazilian coastal Atlantic Forest. J. Biogeogr 42:2389–2401. https://doi.org/10.1111/jbi.12597
Thompson JD, Higgins DG, Gibson TJ (1994) CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22:4673–4680. https://doi.org/10.1093/nar/22.22.4673
Trindade ME, Cetra M, Jucá-Chagas R (2010) Ictiofauna do Ribeirão Limoeiro, Bacia do Rio Cachoeira, BA. Biota Neotrop 4:111–117. https://doi.org/10.1590/s1676-06032010000400015
Velasco A, Aldrey A, Pérez-Martín RI et al (2016) Assessment of the labelling accuracy of spanish semipreserved anchovies products by FINS (forensically informative nucleotide sequencing). Heliyon 2:e00124. https://doi.org/10.1016/j.heliyon.2016.e00124
Ward RD, Hanner R, Hebert PD (2009) The campaign to DNA barcode all fishes, FISH-BOL. J Fish Biol 74:329–356. https://doi.org/10.1111/j.1095-8649.2008.02080.x
Weitzman SH, Menezes NA, Weitzman, MJ (1988) Phylogenetic biogeography of the Glandulocaudini (Teleostei: Characiformes, Characidae) with comments on the distribution of other freshwater fishes in eastern and southeastern Brazil. In: Vanzolini PE, Heyer WR (Eds) Proceedings of a workshop on neotropical distribution patterns, Academia Brasileira de Ciências, Rio de Janeiro, Rio de Janeiro, pp 225–256
Zanata AM, Camelier P (2009) Astyanax vermilion and Astyanax vermilion: new characid fishes (Ostariophysi: Characiformes) from Northeastern Bahia, Brazil. Neotrop Ichthyol 7:175–184. https://doi.org/10.1590/S1679-62252009000200007
Zhang J, Kapli P, Pavlidis P et al (2013) A general species delimitation method with applications to phylogenetic placements. Bioinformatics 29:2869–2876. https://doi.org/10.1093/bioinformatics/btt499
Zink RM, Barrowclough GF (2008) Mitochondrial DNA under siege in avian phylogeography. Mol Ecol 17:2107–2121. https://doi.org/10.1111/j.1365-294x.2008.03737.x
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The authors would like to thank FAPESB (RED0009/2013 and BOL0055/2016) and Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - Brazil (CAPES) (Finance Code 001) for the financial support and SISBIO for the collection license (# 26752).
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This work was supported by CAPES (financial code 001) and FAPESB (RED009/2013 and BOL0055/2016).
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All authors contributed to the study conception and design. Material preparation, data collection and analysis were performed by JLPdS and JdAB. The first draft of the manuscript was written by JLPdS and all authors commented on previous versions of the manuscript. All authors read and approved the final manuscript.
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de Sousa, J.L.P., de Araújo Bitencourt, J., Sampaio, I. et al. “More than meets the eye”: phylogeographic inferences and remarkable cryptic diversity and in endemic catfish Parotocinclus (Loricariidae: Hypoptopomatinae) from neglected and impacted basins in South America. Conserv Genet 22, 411–425 (2021). https://doi.org/10.1007/s10592-021-01336-3
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DOI: https://doi.org/10.1007/s10592-021-01336-3