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Genetic interactions among native and introduced stocks of Oncorhynchus mykiss in the upper Willamette River, Oregon

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Abstract

First recognized as threatened with extinction in 1999, native winter steelhead Oncorhynchus mykiss from Oregon’s Upper Willamette River (UWR) declined to a record-low 543 adult fish in 2017. This anadromous species has been seriously affected by habitat loss caused by impassable dams, intense predation from pinnipeds, and water pollution. Genetic and ecological risks posed by non-native hatchery steelhead may also limit recovery. Here we used 15 microsatellite markers to investigate the genetic composition of naturally-produced juvenile O. mykiss, collected from the most downstream UWR location and other sites throughout the upper basin. Our results, based on 1012 individuals, identified some natural production by summer steelhead released by ongoing hatchery programs, but even greater influence from discontinued non-native winter steelhead hatchery programs. Genetic influence from hatchery programs was nearly absent in above-dam habitats, but concentrated in below-dam habitats and rivers stocked with hatchery steelhead. Although informative, our microsatellite data lacked sufficient power to assign individual fish to hybrid classes, and we urge caution when interpreting similar results from few loci. Taken together, our findings suggest that fish sorting facilities can assist with the conservation of steelhead populations reintroduced above dams, and that hatchery programs can have a lasting genetic influence over naturally-spawning populations, even after stocking has ceased. To conserve the genetic integrity of native UWR steelhead, we recommend that managers consider the lineage of steelhead used for reintroductions, continue to implement policies that limit genetic risks from existing hatchery programs and explore ways to alleviate persistent risks from discontinued programs.

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Data and Supplementary Materials are available at https://doi.org/10.17632/hnp7dsrtdk.2.

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Acknowledgements

We thank Portland General Electric for permission to capture and sample juvenile steelhead at their Sullivan Power Plant (Willamette Falls). This work was made possible through the tremendous field efforts of Oregon State University’s Stream Team, led by Stan Gregory, as well as ODFW staff Eric Bailey, Kersten Schnurle, Luke Whitman, Greg Grenbemer, Brett Boyd, Dan Peck, Kurt Kremers, Mike Hogansen, and many more. We thank Jon Bowers (ODFW) for assistance with ArcGIS and map development, and Matt Falcy (ODFW) for R support and HYBRIDDECTIVE analyses. We thank Bill Marshall (Cascade Timber Consulting), Mark Giustina (Giustina Land and Timber Co.), David Sweeney (Stimson Lumber Co.), Scott Marlega and Denise Lindly (both Weyerhaeuser Company) for providing permits to access streams on private timberlands. Maureen Hess (Columbia River Inter-Tribal Fish Commission) collected data for several baseline populations included in our study. Funding for collection and analysis of samples was provided, in part, by the U.S. Army Corps of Engineers, Portland District (Task Order W9127N-10-02-0008-0015) and the ODFW Restoration and Enhancement Board (Project 13-055). Field work performed by ODFW biologists was conducted with animal handling authorization provided by Oregon Administrative Rule 635-011-0066.

Funding

Financial support for this work was provided by the Oregon Department of Fish and Wildlife (ODFW), the U.S. Army Corps of Engineers, Portland District (Task Order W9127N-10-02-0008-0015) and the ODFW Restoration and Enhancement Board (Project 13-055).

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Authors and Affiliations

  1. Oregon Department of Fish and Wildlife, Corvallis Research Laboratory, Corvallis, OR, 97333, USA

    Marc A. Johnson & Thomas A. Friesen

  2. Manchester Research Station, Northwest Fisheries Science Center, National Marine Fisheries Service, National Oceanic and Atmospheric Administration, 7305 Beach Drive East, Port Orchard, WA, 98366, USA

    Donald M. VanDoornik & David J. Teel

  3. Northwest Fisheries Science Center, National Marine Fisheries Service, National Oceanic and Atmospheric Administration, 2725 Montlake Boulevard East, Seattle, WA, 98112, USA

    James M. Myers

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  1. Marc A. Johnson
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  2. Thomas A. Friesen
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  4. David J. Teel
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  5. James M. Myers
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All authors contributed to study concept, data collection and writing. MAJ prepared the first draft of the manuscript. All authors contributed to and approved the final draft.

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Correspondence to Marc A. Johnson.

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Johnson, M.A., Friesen, T.A., VanDoornik, D.M. et al. Genetic interactions among native and introduced stocks of Oncorhynchus mykiss in the upper Willamette River, Oregon. Conserv Genet 22, 111–124 (2021). https://doi.org/10.1007/s10592-020-01322-1

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