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Effective to census population size ratios in two Near Threatened Mediterranean amphibians: Pleurodeles waltl and Pelobates cultripes

Conservation Genetics volume 18, pages 1201–1211 (2017)Cite this article

Abstract

Efforts to mitigate amphibian declines are hindered by a lack of information about basic aspects of their biology and demography. The effective to census population size ratio (N e /N c ) is one of the most important parameters for the management of wildlife populations because it combines information on population abundance and genetic diversity and helps predict population viability in the long term. Few studies have calculated this ratio in amphibians, which sometimes show low ratios, associated with a higher extinction risk. Here we integrate field-based (capture-mark-recapture studies, egg string counts) and molecular approaches (estimation of the effective number of breeders (N b ) and the effective population size (N e ) based on genotypes from larval cohorts and candidate parents) to produce the first estimates of the N e /N c and N b /N c ratios in two amphibians, the Iberian ribbed newt Pleurodeles waltl and the western spadefoot Pelobates cultripes. Additionally, we investigate sex-biased dispersal in both species based on direct (field observations) and indirect (genetic) evidence. Both species showed similar ratios, slightly lower in Pleurodeles (0.21–0.24) than in Pelobates (0.25–0.30). Observed displacement rates were low in both species (P. waltl = 0.51%; P. cultripes = 1.23%). We found no evidence for sex-biased dispersal in P. cultripes, but both direct and indirect evidences suggest a tendency for female-biased dispersal in P. waltl. We discuss differences in the genetic estimates of N e and N b provided by three inference methods and the implications of our findings for the management of these species, characteristic of Mediterranean wetlands in the Iberian Peninsula and listed as Near Threatened.

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Acknowledgements

We thank J. Agüera, F. Alda, R. Álvarez, S. Barrionuevo, P. Cabezas, M. Casal, E. Garrandés, J.L. Gutiérrez, E. Iranzo, I. Irisarri, D. Llusia, P. Pavón, C. Pedraza, J. Pérez, E. Recuero, G. Rodríguez, A. Sabalza, D. Salvi, L. San José, C. Settanni, I. Urbán and M. Peñalver for help during field work, P. Arntzen for constructive comments on a previous draft, and S. Abalde for help with R scripts for analyses on replicate subsamples. JGR was supported by the Consejo Superior de Investigaciones Científicas of Spain (CSIC) and the European Social Fund (ESF) (JAE-pre PhD fellowship). GSM is funded by a predoctoral grant provided by the Asociación de Amigos de la Universidad de Navarra. This research was funded by grants CGL2008-04271-C02-01/BOS, and CGL2011-28300 (Ministerio de Ciencia e Innovación -MICINN-), Ministerio de Economía y Competitividad -MEC-, Spain, and FEDER) to IMS, who was supported by funding from the Spanish “Ramón y Cajal” and Severo Ochoa (SEV-2012-0262) programs.

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  1. I. Martínez-Solano

    Present address: Departamento de Biodiversidad y Biología Evolutiva, Museo Nacional de Ciencias Naturales, c/ José Gutiérrez Abascal, 2, 28006, Madrid, Spain

Authors and Affiliations

  1. Departamento de Biodiversidad y Biología Evolutiva, Museo Nacional de Ciencias Naturales, CSIC, c/ José Gutiérrez Abascal, 2, 28006, Madrid, Spain

    J. Gutiérrez-Rodríguez & I. Martínez-Solano

  2. Department of Environmental Biology, University of Navarra, Irunlarrea, 1, 31008, Pamplona, Spain

    G. Sánchez-Montes

  3. Instituto de Investigación en Recursos Cinegéticos (IREC-CSIC-UCLM-JCCM), Ronda de Toledo, s/n, 13071, Ciudad Real, Spain

    I. Martínez-Solano

  4. CIBIO/InBIO, Centro de Investigacão em Biodiversidade e Recursos Genéticos da Universidade do Porto, InBIO, Campus Agrário de Vairão, 4485-661, Vairão, Portugal

    I. Martínez-Solano

  5. Ecology, Evolution, and Development Group, Department of Wetland Ecology, Doñana Biological Station, CSIC, c/ Américo Vespucio, s/n, 41092, Seville, Spain

    I. Martínez-Solano

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Gutiérrez-Rodríguez, J., Sánchez-Montes, G. & Martínez-Solano, I. Effective to census population size ratios in two Near Threatened Mediterranean amphibians: Pleurodeles waltl and Pelobates cultripes . Conserv Genet 18, 1201–1211 (2017). https://doi.org/10.1007/s10592-017-0971-5

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Keywords

  • Abundance
  • Capture-mark-recapture
  • Conservation
  • Genetic monitoring
  • Linkage-disequilibrium method
  • Sibship frequency method
  • Sex-biased dispersal