Social corbiculate bees such as honey bees and bumble bees maintain a specific beneficial core microbiome which is absent in wild bees. It has been suggested that maintaining this microbiome can prevent disease and keep bees healthy. The main aim of our study was to identify if there are any core bacterial groups in the non-corbiculate bees Ceratina and Megalopta that have been previously overlooked. We additionally test for associations between the core bee microbes and pollen provisions to look for potential transmission between the two. We identify three enterotypes in Ceratina samples, with thirteen core bacterial phylotypes in Ceratina females: Rosenbergiella, Pseudomonas, Gilliamella, Lactobacillus, Caulobacter, Snodgrassella, Acinetobacter, Corynebacterium, Sphingomonas, Commensalibacter, Methylobacterium, Massilia, and Stenotrophomonas, plus 19 in pollen (6 of which are shared by bees). Unlike Apis bees, whose gut microbial community differs compared to their pollen, Ceratina adults and pollen largely share a similar microbial composition and enterotype difference was largely explained by pollen age. Megalopta displays a highly diverse composition of microbes throughout all adults, yet Lactobacillus and Saccharibacter were prevalent in 90% of adults as core bacteria. Only Lactobacillus was both a core bee and pollen provision microbe in all three species. The consequences of such diversity in core microbiota between bee genera and their associations with pollen are discussed in relation to identifying potentially beneficial microbial taxa in wild bees to aid the conservation of wild, understudied, non-model bee species.
We thank Sean Lombard and Nicholas Pizzi for assistance with nest collections, Krista Ciaccio and Wyatt Shell for nest processing, and Jason Rothman for DNA extractions and library preparation. Funding from the University of California Riverside to QSM, the New Hampshire Agricultural Experiment Station, Tuttle Research Foundation, and the University of New Hampshire to SMR supported this work. Media acknowledgements; Ceratina calcarata (in Fig. 4) photo by J.C. Lucier (CC BY-NC 2.0); Megalopta genalis (in Fig. 6) photo by Sam Droege (CC BY 2.0); Apis mellifera (in Fig. 8) photo by Gustavo Fotoopa (CC BY-NC-ND); Ceratina nest diagram (Fig. 5a) by Wyatt Shell.
Corby-Harris V, Snyder L a, Schwan MR et al (2014b) Origin and effect of Acetobacteraceae Alpha 2.2 in honey bee larvae and description of Parasaccharibacter apium, gen. nov., sp. nov. Appl Environ Microbiol. doi:10.1128/AEM.02043-14PubMedPubMedCentralGoogle Scholar
Engel P, Kwong WK, McFrederick QS, et al (2016) The bee microbiome: impact on bee health and model for evolution and ecology of host-microbe interactions. MBio 7:1–9. doi:10.1128/mBio.02164-15Google Scholar
Graystock P, Goulson D, Hughes WOH (2015) Parasites in bloom: flowers aid dispersal and transmission of pollinator parasites within and between bee species. Proc R Soc B Biol Sci 282:20151371. doi:10.1098/rspb.2015.1371CrossRefGoogle Scholar
McFrederick QS, Wcislo WT, Hout MC, Mueller UG (2014) Host species and developmental stage, but not host social structure, affects bacterial community structure in socially polymorphic bees. FEMS Microbiol Ecol 88:398–406. doi:10.1111/1574-6941.12302CrossRefPubMedGoogle Scholar
Reynolds AP, Richards G, de la Iglesia B, Rayward-Smith VJ (2006) Clustering rules: a comparison of partitioning and hierarchical clustering algorithms. J Math Model Algorithms 5:475–504. doi:10.1007/s10852-005-9022-1CrossRefGoogle Scholar