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Conservation Genetics

, Volume 17, Issue 5, pp 1055–1066 | Cite as

Conservation genetics of the endangered San Francisco Bay endemic salt marsh harvest mouse (Reithrodontomys raviventris)

  • M. J. Statham
  • S. Aamoth
  • L. Barthman-Thompson
  • S. Estrella
  • S. Fresquez
  • L. D. Hernandez
  • R. Tertes
  • B. N. Sacks
Research Article

Abstract

The salt marsh harvest mouse (SMHM, Reithrodontomys raviventris) is an endangered species endemic to the San Francisco Bay region of California, USA, where habitat loss and fragmentation over the past century have reduced the mouse’s distribution to <25 % of its historical range. To aid in conservation prioritization, we first investigated the possibility of hybridization with the morphologically similar western harvest mouse (WHM, R. megalotis) in areas of sympatry and developed genetic tools to differentiate the two species. We then investigated the phylogeography and genetic structure of the SMHM, including support for currently recognized SMHM subspecies designations. Lastly, we evaluated the morphological criteria currently used for the identification of species in the field. Analyses using mtDNA cytochrome b sequences and 11 microsatellites from 142 mice indicated complete and substantial separation of the SMHM and WHM, with no evidence of hybridization. These genetic markers as well as the mtDNA control region also identified a deep genetic division within the SMHM concordant with the current subspecies designations, R. r. raviventris and R. r. halicoetes. We identified the lowest genetic diversity within the southern subspecies, which inhabits a much reduced and highly fragmented portion of the species range. Morphological field identification of harvest mouse species was more successful at identifying SMHM (92 %) than WHM (44 %), with a large portion of WHM being incorrectly identified as SMHM. Field identification of harvest mouse species in the range of the southern SMHM subspecies was just above 50 %, indicating that current methods for morphological differentiation of species in that area are insufficient. Our confirmation of genetically distinct SMHM subspecies highlights the importance of determining the status and genetic composition of relict populations in the remaining patches of marshland in the central San Francisco Bay where the two subspecies may occur, as well as developing better tools for the discrimination of species, particularly in the range of the southern subspecies

Keywords

Field identification Management Morphology Population subdivision Species identification Subspecies 

Notes

Acknowledgments

The primary funding for this research came from California Department of Fish and Wildlife Grant (P1282009). Additional funding came from the Veterinary Genetics Laboratory at UC Davis. Thank you for to Bill Burkhard (DWR), Peter Moyle (UC Davis) for provision of student funding. SMHM survey cooperators include Karen Taylor (DFW Napa/Sonoma Wildlife Area), Stacy Martinelli (DFW, Fagen Marsh), John Krause (DFW, Eden Landing Ecological Reserve), USFWS staff Joy Albertson (Don Edwards SFB NWR), Meg Marriott (USFWS, San Pablo Bay NWR), and Isa Woo (USGS, San Pablo Bay). Thank you to Natalie Goddard, Michelle Holtz, and Sini Reponen for their help in the field and laboratory. Thank you to two anonymous reviewers whose comments and suggestions improved the quality of this paper.

Supplementary material

10592_2016_843_MOESM1_ESM.docx (44 kb)
Supplementary material 1 (DOCX 43 kb)

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Copyright information

© Springer Science+Business Media Dordrecht 2016

Authors and Affiliations

  • M. J. Statham
    • 1
  • S. Aamoth
    • 1
  • L. Barthman-Thompson
    • 2
  • S. Estrella
    • 2
  • S. Fresquez
    • 1
    • 2
  • L. D. Hernandez
    • 1
    • 2
  • R. Tertes
    • 3
  • B. N. Sacks
    • 1
    • 4
  1. 1.Mammalian Ecology and Conservation Unit, Veterinary Genetics LaboratoryUniversity of CaliforniaDavisUSA
  2. 2.Suisun Marsh Unit, Bay Delta RegionCalifornia Department of Fish and WildlifeStocktonUSA
  3. 3.Don Edwards San Francisco Bay National Wildlife RefugeUnited State Fish and Wildlife ServiceFremontUSA
  4. 4.Department of Population Health and Reproduction, School of Veterinary MedicineUniversity of CaliforniaDavisUSA

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