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First identification of interspecies hybridization in the freshwater stingrays Potamotrygon motoro and P. falkneri (Myliobatiformes, Potamotrygonidae)

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Abstract

The stingrays Potamotrygon motoro and P. falkneri occurred in sympatry in the Paraguay, Lower and Middle Paraná River, and belong to distinct ecomorphological groups. The construction of a hydroelectric dam in Itaipu in 1982 facilitated the invasion of Upper Paraná River by both species with unpredictable ecological consequences. The aim of the present work was to evaluate the putative hybridization between these species, using mitochondrial (COI and CytB) and nuclear microsatellite markers. Diagnostic nuclear (Pm 21 and Pm 25 microsatellites) and mitochondrial (Hap1 present in P. motoro, Hap2 and Hap3 present in P. falkneri) DNA markers enabled us the evaluation of hybridization processes. Four individuals morphologically characterized as P. motoro but showing P. falkneri mtDNA haplotypes were suspected to be the result of hybridization and were further analyzed with microsatellites. These individuals showed the combination of diagnostic alleles for both species, thus demonstrating for the first time the occurrence of hybrids between species of stingrays.

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Acknowledgments

This work was supported by Grants from the Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) and the Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq).

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Authors and Affiliations

  1. Laboratório de Biologia e Genética de Peixes, Instituto de Biociências de Botucatu, UNESP, Botucatu, SP, 18618-970, Brazil

    V. P. Cruz, F. F. Mendonça, C. Oliveira & F. Foresti

  2. Laboratori d’Ictiologia Genètica, Departament of Biology, Faculty of Sciences, University of Girona, Campus de Montilivi, 17071, Girona, Spain

    M. Vera

  3. Laboratório Genética Pesqueira e Conservação, Instituto do Mar, Universidade Federal de São Paulo – UNIFESP, Santos, SP, 11030-400, Brazil

    F. F. Mendonça

  4. Departamento de Genètica, Facultad de Veterinaria, Universidad de Santiago de Compostela, 27002, Lugo, Spain

    M. Vera, B. G. Pardo & P. Martinez

Authors
  1. V. P. Cruz
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  2. M. Vera
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  3. F. F. Mendonça
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  4. B. G. Pardo
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  5. P. Martinez
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  6. C. Oliveira
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  7. F. Foresti
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Correspondence to V. P. Cruz.

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Cruz, V.P., Vera, M., Mendonça, F.F. et al. First identification of interspecies hybridization in the freshwater stingrays Potamotrygon motoro and P. falkneri (Myliobatiformes, Potamotrygonidae). Conserv Genet 16, 241–245 (2015). https://doi.org/10.1007/s10592-014-0642-8

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  • DOI: https://doi.org/10.1007/s10592-014-0642-8

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