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Conservation Genetics

, Volume 15, Issue 6, pp 1417–1431 | Cite as

Historical and contemporary forces shape genetic variation in the Olympic mudminnow (Novumbra hubbsi), an endemic fish from Washington State, USA

  • Patrick W. DeHaanEmail author
  • Brice A. Adams
  • Roger A. Tabor
  • Denise K. Hawkins
  • Brad Thompson
Research Article

Abstract

Genetic data have become increasingly useful for conservation planning when data regarding population status and long-term viability is limited. The Olympic mudminnow is the only fish species endemic to Washington State, USA. The species is an increasing priority for conservation given its limited distribution and increasing habitat loss. Presently, information important for developing conservation plans including population abundance data, knowledge of population boundaries, and estimates of gene flow among populations are limited. We used microsatellite markers to assess the level of genetic variation within and among Olympic mudminnow collections from 23 sites across the species range. Genetic variation within collections ranged widely and was greatest within the Chehalis River Basin, a former glacial refugium. Analysis of population boundaries showed that each collection site represented a unique population with the exception of collections made within two large wetland and stream complexes. Genetic variation among populations appears to be strongly influenced by glacial history and the species’ life history. Populations originating from the Chehalis River glacial refugium clustered together in multiple analyses and populations from the Olympic Coast, which persisted in separate refugia and have limited capacity for dispersal, showed a high level of differentiation. Competing theories existed regarding the origins of disjunct populations in east Puget Sound and genetic data showed that these populations represent undocumented introductions rather than a glacial remnant or historic colonization from the Chehalis refugium. Data presented in this study will help fill important information gaps and advance conservation planning for this species.

Keywords

Olympic mudminnow Microsatellites Genetic variation Historic isolation Introduced populations 

Notes

Acknowledgments

Funding for this study was provided by the U.S. Fish and Wildlife Service, Washington Fish and Wildlife Office. We would like to thank the following individuals for assisting with genetic sample collections: Molly Hallock (WDFW), Teal Waterstrat (USFWS), Kira Mazzi (USFWS), Dan Lantz (USFWS), Larry Gilbertson (Quinault Tribe), Pat Trotter (retired), Dan Spencer (USFWS), Hans Berge (King County), Pat Crain (NPS), and John Trobaugh (WDNR). We would also like to thank Teal Waterstrat for producing the study map, Pat Crain for sharing information regarding the history of the James Pond population, Jeanelle Miller and Molly Hallock for sharing information regarding the origins of Olympic mudminnow in east Puget Sound, and Patty Crandell, Christian Smith, and two anonymous reviewers for providing helpful comments on earlier versions of this manuscript. The findings and conclusions in this manuscript are those of the authors and do not necessarily represent the views of the U.S. Fish and Wildlife Service.

Supplementary material

10592_2014_627_MOESM1_ESM.pdf (875 kb)
Supplementary material 1 (PDF 875 kb)

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Copyright information

© Springer Science+Business Media Dordrecht (outside the USA) 2014

Authors and Affiliations

  • Patrick W. DeHaan
    • 1
    Email author
  • Brice A. Adams
    • 1
  • Roger A. Tabor
    • 2
  • Denise K. Hawkins
    • 1
    • 3
  • Brad Thompson
    • 2
  1. 1.U.S. Fish and Wildlife ServiceAbernathy Fish Technology CenterLongviewUSA
  2. 2.U.S. Fish and Wildlife ServiceWashington Fish and Wildlife OfficeLaceyUSA
  3. 3.U.S. Fish and Wildlife ServiceWashington Fish and Wildlife OfficeLaceyUSA

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