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Conservation Genetics

, Volume 15, Issue 4, pp 897–907 | Cite as

Using genetic analysis to estimate population size, sex ratio, and social organization in an Asian elephant population in conflict with humans in Alur, southern India

  • Subhankar Chakraborty
  • D. Boominathan
  • Ajay A. Desai
  • T. N. C. VidyaEmail author
Research Article

Abstract

With growing human and, possibly, elephant populations and a drastic increase in anthropogenic activities, human–elephant conflict in Asia has been on the rise. The Alur area in Karnataka state, southern India, is one such case in point, which has witnessed increasing levels of human–elephant conflict over the last two decades. The tiny, moderately protected habitat available for elephants in this human-dominated landscape does not appear to be able to support elephants over the long term. Options to deal with the escalating conflict include translocation of elephants, bringing elephants into captivity, and culling. We carried out a molecular genetic study of elephants in the Alur area to estimate the minimum number of elephants using the area, the sex ratio, genetic relatedness between individuals, and genetic structure with regard to the larger population in the landscape, so that informed management decisions could be made. Fresh dung samples were collected from the field and genotyped using 12 microsatellite loci. We found 29 unique individuals in the population, comprising 17 females and 12 males of different age classes. Relatedness between females suggested independent colonisations by discrete, small groups rather than by one cohesive clan of related females. This obviates the need for a single solution for dealing with all the females in the area in order to maintain social integrity, and has implications in terms how these elephants can be dealt with. We demonstrate how social organization inferred through molecular data from non-invasive sampling can inform management decisions.

Keywords

Asian elephant Genetic relatedness Hassan district Human–elephant conflict Non-invasive sampling Social organization 

Notes

Acknowledgments

The fieldwork for this study was funded by WWF India, and the labwork by the Department of Science and Technology, Government of India, through the Ramanujan Fellowship to T. N. C. Vidya. Jawaharlal Nehru Centre for Advanced Scientific Research provided infrastructural facilities. We thank the Karnataka Forest Department for permits and field support, especially Mr. Vinaya Chandra, Range Forest Officer, Yesalur, who provided logistic support in the field. Mr. V. Ganesha and forest watchers, Mr. M. S. Guruvappa and Mr. Venkatesh, provided field assistance. We also thank the network of informants, especially Mr. I. D. Channamallikarjuna (of Igoor), who provided useful information of elephant presence at various sites. CA Anitha of the Sequencing Facility at JNCASR helped with genotyping samples. Hansraj Gautam helped with GIS tools. We thank an anonymous reviewer for helpful comments on the manuscript.

Supplementary material

10592_2014_587_MOESM1_ESM.docx (73 kb)
Supplementary material 1 (DOCX 71 kb)

References

  1. Ahlering MA, Hedges S, Johnson A, Tyson M, Schuttler SG, Eggert LS (2011) Genetic diversity, social structure, and conservation value of the elephants of the Nakai Plateau, Lao PDR, based on non-invasive sampling. Conserv Genet 12:413–422CrossRefGoogle Scholar
  2. Appayya MK (1995) Elephants in Karnataka (India): a status report. In: Daniel JC, Datye HS (eds) A week with elephants. Bombay Natural History Society and Oxford University Press, Bombay, pp 88–93Google Scholar
  3. Appayya MK, Desai AA (2007) Assessment of problems caused by elephants in Hassan district, Karnataka state. Report to Project Elephant, Ministry of Environment and Forests, Government of India, and Chief Wildlife Warden, Karnataka Forest Department, KarnatakaGoogle Scholar
  4. Barnes RFW (1996) The conflict between humans and elephants in the Central African forests. Mamm Rev 26:67–80CrossRefGoogle Scholar
  5. Barnes RFW (2002) The problem of precision and trend detection posed by small elephant populations in West Africa. Afr J Ecol 40:179–185CrossRefGoogle Scholar
  6. Barua P (1995) Managing a problem population of elephants. In: Daniel JC, Datye HS (eds) A week with elephants. Bombay Natural History Society and Oxford University Press, Bombay, pp 150–161Google Scholar
  7. Baskaran N, Desai AA (2000) Population and demography of Asian elephant (Elephas maximus) in Mudumalai Wildlife Sanctuary, southern India. Final project report, Bombay Natural History Society and Tamil Nadu Forest Department, IndiaGoogle Scholar
  8. Baskaran N, Balasubramanian S, Swaminathan S, Desai AA (1995) Home range of elephants in the Nilgiris Biosphere Reserve, South India. In: Daniel JC, Datye HS (eds) A week with elephants. Bombay Natural History Society and Oxford University Press, Bombay, pp 296–313Google Scholar
  9. Baskaran N, Varma S, Sar CK, Sukumar R (2011) Current status of Asian elephants in India. Gajah 35:47–54Google Scholar
  10. Bist SS (2002) An overview of elephant conservation in India. Indian For 128:121–136Google Scholar
  11. Charif RA, Ramey RR, Langbauer WR, Payne KB, Martin RB, Brown LM (2005) Spatial relationships and matrilineal kinship in African savanna elephant (Loxodonta africana) clans. Behav Ecol Sociobiol 57:327–338CrossRefGoogle Scholar
  12. Comstock KE, Wasser SK, Ostrander EA (2000) Polymorphic microsatellite DNA loci identified in the African elephant (Loxodonta africana). Mol Ecol 9:1004–1006PubMedCrossRefGoogle Scholar
  13. Desai A, Johnsingh AJT (1995) Social organization and reproductive strategy of the male Asian elephant (Elephas maximus). In: Daniel JC, Datye HS (eds) A week with elephants. Bombay Natural History Society and Oxford University Press, Bombay, p 532Google Scholar
  14. Desai AA, Vidya TNC, Boominathan D, Chakraborty S, Mohanraj N (2013) Composition of the elephant population in Kattepura area and its movement pattern. Report submitted to the Karnataka Forest Department, BangaloreGoogle Scholar
  15. Eggert LS, Eggert JA, Woodruff DS (2003) Estimating population sizes for elusive animals: the forest elephants of Kakum National Park, Ghana. Mol Ecol 12:1389–1402PubMedCrossRefGoogle Scholar
  16. Elouard C (2000) Transformation and degradation of natural forests. In: Ramakrishnan PS, Chandrashekara UM, Elouard C, Guilmoto CZ, Maikhuri RK, Rao KS, Saxena KG, Shankar S (eds) Mountain biodiversity, land use dynamics, and traditional ecological knowledge. Oxford and IBH Publishing Company, New Delhi, pp 70–73Google Scholar
  17. Evanno G, Regnaut S, Goudet J (2005) Detecting the number of clusters of individuals using the software STRUCTURE: a simulation study. Mol Ecol 14:2611–2620PubMedCrossRefGoogle Scholar
  18. Evett IW, Weir BS (1998) Interpreting DNA evidence: statistical genetics for forensic scientists. Sinauer Associates, SunderlandGoogle Scholar
  19. Excoffier L, Laval G, Schneider S (2005) ARLEQUIN ver. 3.0: an integrated software package for population genetics data analysis. Evol Bioinform 1:47–50Google Scholar
  20. Fernando P, Lande R (2000) Molecular genetic and behavioural analysis of social organization in the Asian elephant (Elephas maximus). Behav Ecol Sociobiol 48:84–91CrossRefGoogle Scholar
  21. Fernando P, Melnick DJ (2001) Molecular sexing eutherian mammals. Mol Ecol Notes 1:350–353CrossRefGoogle Scholar
  22. Fernando P, Vidya TNC, Melnick DJ (2001) Isolation and characterization of tri- and tetranucleotide microsatellite loci in the Asian elephant, Elephas maximus. Mol Ecol Notes 1:232–233CrossRefGoogle Scholar
  23. Fernando P, Vidya TNC, Rajapakse C, Dangolla A, Melnick DJ (2003) Reliable non-invasive genotyping: fantasy or reality? J Hered 94:115–123PubMedCrossRefGoogle Scholar
  24. Fernando P, Leimgruber P, Prasad T, Pastorini J (2012) Problem-elephant translocation: translocating the problem and the elephant? PLoS One 7:e50917PubMedCentralPubMedCrossRefGoogle Scholar
  25. Gray TNE, Vidya TNC, Potdar S, Bharti DK, Sovanna P (2014) Population size estimation of an Asian elephant population in eastern Cambodia through non-invasive mark-recapture sampling. Conserv Genet. doi: 10.1007/s10592-014-0579-y
  26. Hedges S, Fisher K, Rose R (2008) Range-wide mapping workshop for Asian elephants (Elephas maximus). Report to US Fish and Wildlife Service, CambodiaGoogle Scholar
  27. Hedges S, Johnson A, Ahlering M, Tyson M, Eggert LS (2013) Accuracy, precision, and cost-effectiveness of conventional dung density and fecal DNA based survey methods to estimate Asian elephant (Elephas maximus) population size and structure. Biol Conserv 159:101–108CrossRefGoogle Scholar
  28. Hedrick P (1995) Gene flow and genetic restoration: the Florida panther as a case study. Conserv Biol 9:996–1007CrossRefGoogle Scholar
  29. Hubisz MJ, Falush D, Stephens M, Pritchard JK (2009) Inferring weak population structure with the assistance of sample group information. Mol Ecol Res 9:1322–1332CrossRefGoogle Scholar
  30. Jones OR, Wang J (2010) COLONY: a program for parentage and sibship inference from multilocus genotype data. Mol Ecol Res 10:551–555CrossRefGoogle Scholar
  31. Karnataka Elephant Task Force (2012) Report of the Karnataka Elephant Task Force. Report submitted to the High Court of Karnataka, BangaloreGoogle Scholar
  32. Kongrit C, Siripunkaw C, Brockelman WY, Akkarapatumwong V, Wright TF, Eggert LS (2008) Isolation and characterization of dinucleotide microsatellite loci in the Asian elephant (Elephas maximus). Mol Ecol Res 8:175–177CrossRefGoogle Scholar
  33. Lahiri-Choudhury DK (1993) Problems of wild elephant translocation. Oryx 27:53–55CrossRefGoogle Scholar
  34. Leimgruber P, Gagnon JB, Wemmer C, Kelly DS, Songer MA, Selig ER (2003) Fragmentation of Asia’s remaining wildlands: implications for Asian elephant conservation. Anim Conserv 6:347–359CrossRefGoogle Scholar
  35. Lötter HPP, Henley M, Fakir S, Pickover M, Ramose M (2008) Ethical considerations in elephant management. In: Scholes RJ, Mennell KG (eds) Elephant management: a scientific assessment of South Africa. Witwatersrand University Press, Johannesburg, pp 307–338Google Scholar
  36. Madel MJ (1996) Rocky mountain front grizzly bear management program: four year progress report. Montana Department of Fish, Wildlife and Parks, Helena, MontanaGoogle Scholar
  37. Naughton-Treves L (1997) Farming the forest edge: vulnerable places and people around Kibale National Park, Uganda. Geog Rev 87:27–46CrossRefGoogle Scholar
  38. Nyakaana S, Arctander P (1998) Isolation and characterisation of microsatellite loci in the African elephant, Loxodonta africana. Mol Ecol 7:1436–1437PubMedGoogle Scholar
  39. Nyakaana S, Abe EL, Arctander P, Siegismund HR (2001) DNA evidence for social behaviour breakdown in Queen Elizabeth National Park, Uganda. Anim Conserv 4:231–237CrossRefGoogle Scholar
  40. Paetkau D, Strobeck C (1994) Microsatellite analysis of genetic variation in black bear populations. Mol Ecol 3:489–495PubMedCrossRefGoogle Scholar
  41. Pinter-Wollman N (2009) Spatial behaviour in translocated African elephants (Loxodonta africana) in a novel environment: using behaviour to inform conservation. Behaviour 146:1171–1192CrossRefGoogle Scholar
  42. Pinter-Wollman N (2012) Human–elephant conflict in Africa: the legal and political viability of translocations, wildlife corridors, and transfrontier parks for large mammal conservation. J Int Wildl Law Policy 15:152–166CrossRefGoogle Scholar
  43. Pinter-Wollman N, Isbell LA, Hart LA (2009) Assessing translocation outcome: comparing behavioural and physiological aspects of translocated and resident African elephants (Loxodonta africana). Biol Conserv 142:1116–1124CrossRefGoogle Scholar
  44. Pritchard JK, Stephens M, Donnelly P (2000) Inference of population structure using multilocus genotype data. Genetics 155:945–959PubMedCentralPubMedGoogle Scholar
  45. Queller DC, Goodnight KF (1989) Estimating relatedness using genetic markers. Evolution 43:258–275CrossRefGoogle Scholar
  46. Rangarajan M, Desai A, Sukumar R, Easa PS, Menon V, Vincent S, Ganguly S, Talukdar BK, Singh B, Mudappa D, Chowdhary S, Prasad AN (2010) Gajah: securing the future for elephants in India. Report of the Elephant Task Force, Ministry of Environment and Forests, IndiaGoogle Scholar
  47. Rousset F (2008) Genepop’007: a complete reimplementation of the Genepop software for Windows and Linux. Mol Ecol Res 8:103–106CrossRefGoogle Scholar
  48. Sillero-Zubiri C, Sukumar R, Treves A (2007) Living with wildlife: the roots of conflict and the solutions. In: Macdonald DW, Service K (eds) Key topics in conservation biology. Blackwell Publishing, Oxford, pp 253–270Google Scholar
  49. Slotow R, van Dyk G (2004) Ranging of older male elephants introduced to an existing small population without older males: Pilanesberg National Park. Koedoe 47:91–104CrossRefGoogle Scholar
  50. Slotow R, Garaï ME, Reilly BK, Page BR, Carr RD (2005) Population dynamics of elephants re-introduced to small fenced reserves in South Africa. S Afr J Wildl Res 35:23–32Google Scholar
  51. Slotow R, Whyte I, Hofmeyr M, Kerley GHI, Conway T, Scholes TJ (2008) Lethal management of elephant. In: Scholes RJ, Mennell KG (eds) Elephant management: A scientific assessment of South Africa. Witwatersrand University Press, Johannesburg, pp 278–306Google Scholar
  52. Srinivasaiah NS, Sinha A (2012) Rapid assessment of the status of elephants in the conflict ridden areas of Hassan. Unpublished report. National Institute of Advanced Studies, BangaloreGoogle Scholar
  53. Stüwe M, Abdul JB, Nor BM, Wemmer CM (1998) Tracking the movements of translocated elephants in Malaysia using satellite telemetry. Oryx 30:68–74CrossRefGoogle Scholar
  54. Taberlet P, Camarra J-J, Griffin S, Uhrès E, Hanotte O, Waits LP, Dubois-Paganon C, Burke T, Bouvet J (1997) Noninvasive genetic tracking of the endangered Pyrenean brown bear population. Mol Ecol 6:869–976PubMedCrossRefGoogle Scholar
  55. Thirgood S, Woodroffe R, Rabinowitz A (2005) The impact of human–wildlife conflict on human lives and livelihoods. In: Woodroffe R, Thirgood S, Rabinowitz A (eds) People and Wildlife: Conflict or Coexistence?. Cambridge University Press, Cambridge, pp 13–26Google Scholar
  56. Treves A, Jurewicz RR, Naughton-Treves L, Rose RA, Willging RC, Wydeven AP (2002) Wolf depredation on domestic animals: control and compensation in Wisconsin, 1976–2000. Wild Soc Bull 30:231–241Google Scholar
  57. Tyson M, Hedges S, Sitompul AF (2002) WCS-Indonesia Program Sumatran elephant project: six-month report January–June 2002. Unpublished report to the Wildlife Conservation Society, New YorkGoogle Scholar
  58. Van Oosterhout C, Hutchinson WF, Wills DPM, Shipley P (2004) Micro-Checker: software for identifying and correcting genotyping errors in microsatellite data. Mol Ecol Notes 4:535–538CrossRefGoogle Scholar
  59. Vidya TNC, Sukumar R (2005) Social organization of the Asian elephant (Elephas maximus) inferred from microsatellite DNA. J Ethol 23:205–210CrossRefGoogle Scholar
  60. Vidya TNC, Kumar VR, Arivazhagan C, Sukumar R (2003) Application of molecular sexing to free-ranging Asian elephant (Elephas maximus) populations in southern India. Curr Sci 85:1074–1077Google Scholar
  61. Vidya TNC, Fernando P, Melnick JD, Sukumar R (2005) Population differentiation within and among Asian elephant (Elephas maximus) populations in southern India. Heredity 94:71–80PubMedCrossRefGoogle Scholar
  62. Vidya TNC, Varma S, Dang NX, Van Thanh T, Sukumar R (2007) Minimum population size, genetic diversity, and social structure of the Asian elephant in Cat Tien National Park and its adjoining areas, Vietnam, based on molecular genetic analyses. Conserv Genet 8:1471–1478CrossRefGoogle Scholar
  63. Wagner HW, Sefc KM (1999) IDENTITY 1.0. Centre for Applied Genetics, University of Agricultural Sciences, ViennaGoogle Scholar
  64. Wang J (2008) COLONY 2.0.5.0 software. http://www.zsl.org/science/research-projects/software/. Accessed 22 Feb 2014
  65. Wang J (2011) COANCESTRY: a program for simulating, estimating and analysing relatedness and inbreeding coefficients. Mol Ecol Res 11:141–145CrossRefGoogle Scholar
  66. Wilson G, Desai AA, Sim DA, Linklater WL (2013) The influence of the invasive weed Lantana camara on elephant habitat use in Mudumalai Tiger Reserve, southern India. J Trop Ecol 29:199–207CrossRefGoogle Scholar
  67. Woodroffe R, Thirgood S, Rabinowitz A (2005) The impact of human–wildlife conflict on natural systems. In: Woodroffe R, Thirgood S, Rabinowitz A (eds) People and wildlife: conflict or coexistence?. Cambridge University Press, Cambridge, pp 1–12CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media Dordrecht 2014

Authors and Affiliations

  • Subhankar Chakraborty
    • 1
    • 2
  • D. Boominathan
    • 3
  • Ajay A. Desai
    • 4
  • T. N. C. Vidya
    • 1
    Email author
  1. 1.Evolutionary and Organismal Biology UnitJawaharlal Nehru Centre for Advanced Scientific ResearchBangaloreIndia
  2. 2.Theoretical Ecology and Evolution LaboratoryCentre for Ecological Sciences, Indian Institute of ScienceBangaloreIndia
  3. 3.WWF–India, Western Ghats Nilgiris LandscapeGudalurIndia
  4. 4.WWF–India and WWF–InternationalBelgaumIndia

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