Abstract
The application of genetic approaches has enhanced the identification of cryptic species in a wide variety of taxa, often with immediate conservation implications. Here, we employed multilocus DNA analyses to assess genetic variation and its correspondence to taxonomy within the Mealy Amazon (Amazona farinosa), a parrot species found in Central and South America. DNA sequence data from four mitochondrial regions and two nuclear introns were used to infer relationships among all five named subspecies in this species complex. Two reciprocally monophyletic groups with strong nodal support were found; one comprised of the two Central American subspecies guatemalae and virenticeps and one including all three South American subspecies farinosa, chapmani, and inornata. Molecular characters diagnosed distinct Central American and South American lineages, with an estimated divergence time of 1.75–2.70 million years ago as inferred from cytochrome-b (3.5–5.4 % corrected distance). Our data support recognizing Central American and South American Mealy Amazons as separate species worthy of independent conservation management. Furthermore, our results suggest recognition of two separate management units within the South American clade, although further study is required. These findings have important conservation implications as Central American A. farinosa are under increased pressure from habitat destruction and collection for the pet trade, yet are listed as of Least Concern due to their current classification as subspecies’ subsumed within the species complex.
References
Bickford D, Lohman DJ, Sodhi NS, Ng PKL, Meier R, Winker K, Ingram KK, Das I (2007) Cryptic species as a window on diversity and conservation. Trends Ecol Evol 22:148–155
Birdlife International (2004) Threatened birds of the world 2004. Birdlife International, Cambridge
Boessenkool S, Austin JJ, Worthy TH, Scofield P, Cooper A, Seddon PJ, Waters JM (2009) Relict or colonizer? Extinction and range expansion of penguins in southern New Zealand. Proc R Soc B 276:815–821
Burbidge ML, Colbourne RM, Robertson HA, Baker AJ (2003) Molecular and other biological evidence supports the recognition of at least three species of brown kiwi. Conserv Genet 4:167–177
Coates AG (1997) The forging of Central America. In: Coates AG (ed) Central America: a natural history. Yale University Press, New Haven, pp 1–37
Collar NJ, Pittman AJ (1996) Amazona kawalli is a valid name for a valid species. Bull Br Ornithol Club 116:256–265
Cracraft J (1983) Species concepts and speciation analysis. Curr Ornithol 1:159–187
Davis JI, Nixon KC (1992) Populations, genetic-variation, and the delimitation of phylogenetic species. Syst Biol 41:421–435
Eberhard JR, Bermingham E (2004) Phylogeny and biogeography of the Amazona ochrocephala (Aves: Psittacidae) complex. Auk 121:318–332
Eberhard JR, Bermingham E (2005) Phylogeny and comparative biogeography of Pionopsitta parrots and Pteroglossus toucans. Mol Phylogenet Evol 36:288–304
Forshaw J (2010) Parrots of the world. Princeton University Press, Princeton
Hazevoet CJ (1996) Conservation and species lists: taxonomic neglect promotes the extinction of endemic birds, as exemplified by taxa from the eastern Atlantic islands. Bird Conserv Int 6:181–196
Helaers R, Milinkovitch MC (2010) MetaPIGA v2.0: maximum likelihood large phylogeny estimation using the metapopulation genetic algorithm and other stochastic heuristics. BMC Bioinformatics 11:379
Huelsenbeck JP, Ronquist F (2001) MRBAYES: Bayesian inference of phylogenetic trees. Bioinformatics 17:754–755
IUCN (2011) IUCN Red list of threatened species. IUCN. http://iucnredlist.org. Accessed 17 Nov 2011
Johns GC, Avise JC (1998) A comparative summary of genetic distances in the vertebrates from the mitochondrial cytochrome b gene. Mol Biol Evol 15:1481–1490
Juniper T, Parr M (1998) Parrots: a guide to parrots of the world. Yale University Press, New Haven
Kimura M (1980) A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide-sequences. J Mol Evol 16:111–120
Larkin MA, Blackshields G, Brown NP, Chenna R, McGettigan PA, McWilliam H, Valentin F, Wallace IM, Wilm A, Lopez R, Thompson JD, Gibson TJ, Higgins DG (2007) Clustal W and clustal X version 2.0. Bioinformatics 23:2947–2948
Librado P, Rozas J (2009) DnaSP v5: a software for comprehensive analysis of DNA polymorphism data. Bioinformatics 25:1451–1452
Lovette IJ (2004) Mitochondrial dating and mixed-support for the “2 % rule” in birds. Auk 121:1–6
Mace GM (2004) The role of taxonomy in species conservation. Philos Trans Roy Soc B 359:711–719
Murphy SA, Joseph L, Burbidge AH, Austin J (2011) A cryptic and critically endangered species revealed by mitochondrial DNA analyses: the western ground parrot. Conserv Genet 12:595–600
Russello MA, Amato G (2004) A molecular phylogeny of Amazona: implications for Neotropical parrot biogeography, taxonomy, and conservation. Mol Phylogenet Evol 30:421–437
Russello MA, Glaberman S, Gibbs JP, Marquez C, Powell JR, Caccone A (2005) A cryptic taxon of Galapagos tortoise in conservation peril. Biol Lett 1:287–290
Russello MA, Stahala C, Lalonde D, Schmidt KL, Amato G (2010) Cryptic diversity and conservation units in the Bahama parrot. Conserv Genet 11:1809–1821
Salvadori T (1891) Catalogue of the birds in the British museum, vol 20. Trustees of British Museum, London
Stresemann E (1924) Eine Amazona farinosa (Boddaert) mit roter schwanz-bais. Mitt Ornithol Ver Wien 32:43–44
Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S (2011) MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 28:2731–2739
Tarr CL, Fleischer RC (1993) Mitochondrial-DNA variation and evolutionary relationships in the amakihi complex. Auk 110:825–831
Weir JT, Bermingham E, Schluter D (2009) The great American biotic interchange in birds. Proc Natl Acad Sci USA 106:21737–21742
Acknowledgments
We are grateful to the following institutions for providing samples: Academy of Natural Sciences, Philadelphia; Field Museum of Natural History; Louisiana State University Museum of Natural Sciences; Royal Ontario Museum; Smithsonian National Museum of Natural History; University of Kansas Natural History Museum. We also thank J Gilardi for useful comments on this manuscript and K Wenner-Sherrell for assistance in developing figures. The World Parrot Trust provided funding for this research.
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Wenner, T.J., Russello, M.A. & Wright, T.F. Cryptic species in a Neotropical parrot: genetic variation within the Amazona farinosa species complex and its conservation implications. Conserv Genet 13, 1427–1432 (2012). https://doi.org/10.1007/s10592-012-0364-8
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DOI: https://doi.org/10.1007/s10592-012-0364-8