Ten polymorphic microsatellite loci were developed for Graellsia isabelae. Polymorphism was assessed for 20 individuals from a Spanish population (Els-Ports-de-Beseit, Catalonia) and 39 more individuals from one population in the French Alps and six other Spanish localities. Overall, the number of alleles per locus ranged from 5 to 24. Els-Ports-de-Beseit showed an average number of alleles per locus of 9.80 (SD = 4.32), observed heterozygosity was 0.71 (SD = 0.226), and expected heterozygosity was 0.788 (SD = 0.146). Genotypic frequencies conformed to Hardy–Weinberg equilibrium at the Catalonian population, and no evidence for linkage disequilibrium was observed. Multilocus genotypes resulting from this set of markers will be useful to determine genetic diversity and differentiation within and among populations of this highly protected moth. Several loci amplified and resulted polymorphic in two related species: two loci in Actias neidhoeferi, and three loci in A. luna.
This is a preview of subscription content, log in to check access.
Buy single article
Instant access to the full article PDF.
Price includes VAT for USA
Subscribe to journal
Immediate online access to all issues from 2019. Subscription will auto renew annually.
This is the net price. Taxes to be calculated in checkout.
Armour JA, Neumann R, Gobert S, Jeffreys AJ (1994) Isolation of human simple repeat loci by hybridization selection. Hum Mol Genet 3:599–605. doi:10.1093/hmg/3.4.599
Fernández Vidal EH (1992) Comentarios acerca de la distribución geográfica francesa y notas taxonómicas sobre Graellsia isabelae (Graells, 1849) (Lepidoptera: Saturniidae). SHILAP Revta lepid 20:29–49
Gautschi B, Tenzer I, Müller JP, Schmid B (2000a) Isolation and characterization of microsatellite loci in the bearded vulture (Gypaetus barbatus) and crossamplification in three old world vulture species. Mol Ecol 9:2193–2195. doi:10.1046/j.1365-294X.2000.105321.x
Gautschi B, Widmer A, Koella J (2000b) Isolation and characterization of microsatellite loci in the dice snake (Natrix tessellata). Mol Ecol 9:2191–2193. doi:10.1046/j.1365-294X.2000.105320.x
Goudet J (2001) FSTAT, a program to estimate and test gene diversities and fixation indices (version 2.9.3). Available at www.unil.ch/izea/softwares/fstat.html. Accessed 5 Feb 2009
IUCN (2008) IUCN red list of threatened species. Available at www.iucnredlist.org. Accessed 23 Jan 2009
Park SDE (2001) Trypanotolerance in West African cattle and the population genetic effects of selection. PhD thesis, University of Dublin
Van Oosterhout C, Hutchinson WF, Wills DPM, Shipley P (2004) MICRO-CHECKER: software for identifying and correcting genotyping errors in microsatellite data. Mol Ecol Notes 4:535–538. doi:10.1111/j.1471-8286.2004.00684.x
Vila M, Auger-Rozenberg MA, Goussard F, Lopez-Vaamonde C (2009) Effect of non-lethal sampling on life-history traits of the protected moth Graellsia isabelae (Lepidoptera: Saturniidae). Ecol Entomol (in press). doi:10.1111/j.1365-2311.2008.01084.x
Wagner HW, Sefc KM (1999) IDENTITY 1.0. Centre for applied genetics, University of Agricultural Sciences, Vienna. Available at www.uni-graz.at/~sefck/ Accessed 5 Feb 2009
Zhang Z, Schwartz S, Wagner L, Miller W (2000) A greedy algorithm for aligning DNA sequences. J Comput Biol 7:203–214. doi:10.1089/10665270050081478
John Ciseski provided us with samples of Actias luna. Nuria Remon kindly helped us in the lab. We thank J. Casaponsa, A. Cervelló, Y.-L. Chen, F. Goussard, S. Ibáñez, R. Maciá, J. Martí, MICROFAUNA, T. Latasa, J. Nevado, A. Sáez, L.-C. Shih, W.-C. Tsao, and J. Ylla for their help during fieldwork. David Lees improved an earlier version of the manuscript with his comments. We appreciate the support provided by Ecogenics GmbH, Parc Natural dels Ports, and Departament de Medi Ambient (Generalitat de Catalunya). This work was performed under permits from all environmental authorities concerned. It was financially supported by the Spanish Ministry of Education and Science (CGL2007-63549/BOS). NMM was funded by a FPI grant (BES-2008-002571). MV was funded by Xunta de Galicia (Isidro Parga Pondal programme) and European Science Foundation (CONGEN short-visit (1635) and exchange (1683) grants). Sampling of Actias neidhoeferi was supported by National Science Council (97-2621-B-110-001-MY3).
About this article
Cite this article
Vila, M., Marí-Mena, N., Yen, S. et al. Characterization of ten polymorphic microsatellite markers for the protected Spanish moon moth Graellsia isabelae (Lepidoptera: Saturniidae). Conserv Genet 11, 1151–1154 (2010). https://doi.org/10.1007/s10592-009-9905-1
- Graellsia isabelae
- Conservation genetics