Abstract
We isolated and characterized eight microsatellite markers for Dictyochloropsis reticulata, the primary photosynthetic partner of the epiphytic lichen Lobaria pulmonaria. These are the first microsatellite loci reported for a lichen symbiotic alga. These polymorphic markers will be useful for investigating spatial genetic structure, biogeography and dispersal of this eukaryotic alga and will generally shed light on the coevolution of the green-algal lichen symbioses.
References
Armour JA, Neumann R, Gobert S, Jeffreys AJ (1994) Isolation of human simple repeat loci by hybridization selection. Hum Mol Genet 3:599–605. doi:10.1093/hmg/3.4.599
Beck A, Koop H-U (2001) Analysis of the photobiont population in lichens using a single-cell manipulator. Symbiosis 31:57–67
Brodo IM, Duran Sharnoff S, Sharnoff S (2001) Lichens of North America. Yale University Press, London
Gauslaa Y (1994) Lobaria pulmonaria, an indicator of species-rich forests of long ecological continuity. Blyttia 52:119–128
Gautschi B, Tenzer I, Müller JP, Schmid B (2000a) Isolation and characterization of microsatellite loci in the bearded vulture (Gypaetus barbatus) and cross-amplification in three Old World vulture species. Mol Ecol 9:2193–2195. doi:10.1046/j.1365-294X.2000.105321.x
Gautschi B, Widmer A, Koella J (2000b) Isolation and characterization of microsatellite loci in the Dice Snakje (Natrix tessellata). Mol Ecol 3:233–235
Geitler L (1966) Die Chlorococcalen Dictyochloris und Dictyochloropsis. Österreich Bot Zeitschr 113:155–164
Nei M (1987) Molecular evolutionary genetics. Columbia University Press, New York
Raymond M, Rousset F (1995) GENEPOP (version 1.2): population genetics software for exact tests and ecumenicism. J Hered 86:248–249
Rozen S, Skaletsky H (2000) PRIMER3 on the WWW for general users and for biologist programmers. In: Krawetz S, Misener S (eds) Bioinformatics methods and protocols in the series methods in molecular biology. Humana Press, Totowa, pp 365–386
Walser JC, Sperisen C, Soliva M, Scheidegger C (2003) Fungus-specific microsatellite primers of lichens: application for the assessment of genetic variation on different spatial scales in Lobaria pulmonaria. Fungal Genet Biol 40(1):72–82. doi:10.1016/S1087-1845(03)00080-X
Walser JC, Holderegger R, Gugerli F, Hoebee SE, Scheidegger C (2005) Microsatellites reveal regional population differentiation and isolation in Lobaria pulmonaria, an epiphytic lichen. Mol Ecol 14(2):457–467. doi:10.1111/j.1365-294x.2004.02423.x
Werth S, Gugerli F, Holderegger R, Wagner HH, Csencsics D, Scheidegger C (2007) Landscape-level gene flow in Lobaria pulmonaria, an epiphytic lichen. Mol Ecol 16:2807–2815. doi:10.1111/j.1365-294X.2007.03344.x
Wirth V, Scholler H, Scholz P et al (1996) Rote Liste der Flechten (Lichenes) der Bundesrepublik Deutschland. Schriftenr Vegetationsk 28:307–368
Yoshimura I (1998) Lung lichens and their vegetation in Japan and other regions. In: Kondratyuk SY, Coppins BJ (eds) Lobarion Lichens as indicators of the primeval forests of the Eastern Carpathians. Ukrainian Phytosociological Centre, Kiev, pp 53–63
Acknowledgments
We thank C Cornejo for laboratory assistance, R Holderegger for valuable comments on the manuscript and T Terzani for his never-ending support. We also thank the Genetic Diversity Center at ETH Zürich, Switzerland (CCED-GDC) for technical assistance. We acknowledge financial support from the Swiss National Science Foundation (projects 31003A-105830 and 31003A-127346 to Christoph Scheidegger).
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Dal Grande, F., Widmer, I., Beck, A. et al. Microsatellite markers for Dictyochloropsis reticulata (Trebouxiophyceae), the symbiotic alga of the lichen Lobaria pulmonaria (L.). Conserv Genet 11, 1147–1149 (2010). https://doi.org/10.1007/s10592-009-9904-2
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DOI: https://doi.org/10.1007/s10592-009-9904-2