Abstract
Twenty polymorphic microsatellite loci were isolated from the Japanese scallop (Patinopecten yessoensis) using a method of hybridizing twice. The genetic diversity was analyzed by these microsatellite markers in field specimens from Zhangzidao Island in Liaoning Province, China. Observed heterozygosity (H o), expected heterozygosity (H e), number of effective alleles (N e), and polymorphism information contents (PIC) were calculated. The heterozygote deficiency or excess was detected by the fixation index (Fis). The Hardy–Weinberg equilibrium was tested by possibilities (P HWE) using chi-square test. Results indicate that the average of allele numbers was 6.0. The mean values of the parameters H o, H e, N e, and PIC were 0.7921, 0.7076, 4.4347, and 0.6684, respectively. Only 4 of 20 microsatellite markers showed heterozygote deficiency including loci FJ262370, FJ262381, FJ262384, and FJ262400. The P HWE value indicates the wild population deviated somewhat from the Hardy–Weinberg equilibrium. These newly isolated markers increase the available molecular resources of the Japanese scallop.
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Acknowledgments
We gratefully acknowledge the assistance of Key Laboratory faculties (Key Laboratory of Finfish Bioengineering and Breeding of North China, Ministry of Agriculture, The People’s Republic of China). We would like to thank Dr. Zhao Yingying for technical assistance and helpful discussions. This research work was supported by The Project of National Natural Science Foundation of China (30671594), Key Project of the National High Technology Research and Development Program (“863”Program) of China (2006AA10A408), and Ministry of Education New Century Excellent Talents in University (NCET-05-0299).
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Chen, M., Chang, YQ., Ding, J. et al. Isolation and characterization of new microsatellite markers from the Japanese scallop (Patinopecten yessoensis). Conserv Genet 11, 1139–1142 (2010). https://doi.org/10.1007/s10592-009-9900-6
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DOI: https://doi.org/10.1007/s10592-009-9900-6