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Isolation and characterization of polymorphic microsatellite markers in the grasshopper Mioscirtus wagneri (Orthoptera: Acrididae)

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Abstract

Eight polymorphic microsatellite markers were developed for the grasshopper Mioscirtus wagneri. Polymorphism at these loci was evaluated in 25 individuals from Central Spain. The number of alleles per locus ranged from 3 to 9 and their observed and expected heterozygosities ranged from 0.28 to 0.80 and 0.25 to 0.84, respectively. All genotypic frequencies conformed to Hardy–Weinberg equilibrium expectations, with no evidence of genotypic linkage disequilibrium between any pair of loci. These loci will be highly useful for the study of the population genetic structure and diversity of this grasshopper species forming highly fragmented populations of great conservation concern.

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Acknowledgments

This work received financial support from the projects PCI08-0130-3954 (JCCM) and CGL2005-05611-C02-02/BOS (MEC). Specimens were captured under license from the Junta de Comunidades de Castilla-La Mancha (JCCM).

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Authors and Affiliations

  1. Grupo de Investigación de la Biodiversidad Genética y Cultural, Instituto de Investigación en Recursos Cinegéticos—IREC (CSIC, UCLM, JCCM), Ronda de Toledo s/n, 13005, Ciudad Real, Spain

    Maria Pilar Aguirre & Pedro J. Cordero

  2. Departamento de Ecología Evolutiva, Museo Nacional de Ciencias Naturales (CSIC), C/José Gutiérrez Abascal 2, 28006, Madrid, Spain

    Paul Bloor, Ursula Ramírez-Escobar & Joaquín Ortego

Authors
  1. Maria Pilar Aguirre
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  2. Paul Bloor
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  3. Ursula Ramírez-Escobar
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  4. Joaquín Ortego
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  5. Pedro J. Cordero
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Corresponding author

Correspondence to Joaquín Ortego.

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Aguirre, M.P., Bloor, P., Ramírez-Escobar, U. et al. Isolation and characterization of polymorphic microsatellite markers in the grasshopper Mioscirtus wagneri (Orthoptera: Acrididae). Conserv Genet 11, 1119–1121 (2010). https://doi.org/10.1007/s10592-009-9895-z

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  • DOI: https://doi.org/10.1007/s10592-009-9895-z

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