Ten new microsatellite loci for analysis of genetic diversity in isolated populations of the Alpine land snail Cylindrus obtusus

Abstract

In this study we characterized 10 polymorphic microsatellite markers for the land snail Cylindrus obtusus, an endemism of the Austrian Alps with a distribution in isolated populations above approximately 1,600 m. The microsatellite loci were analyzed in 44 individuals from two populations. Number of alleles per locus ranged between two and eight. Observed heterozygosity ranged between 0.00 and 1.00, and expected heterozygosity between 0.09 and 0.72. No significant linkage disequilibrium was found between pairs of loci. One of the sampled populations (Dachstein) showed no deviation from Hardy–Weinberg equilibrium and no presence of null alleles, whereas the other one (Schneeberg) did. These diverging results probably reflect differences in population structure rather than characteristics of the microsatellite loci and underline the usefulness of these markers for studying genetic diversity, population structure and differentiation in C. obtusus.

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Acknowledgments

We are grateful to Helmut Sattmann who initiated the work with C. obtusus and collected the snails. We thank Barbara Däubl and Simone Latkolik for technical assistance, Christian Stauffer for providing the library enrichment facilities and W. Pinsker and E. Haring for providing useful comments on the manuscript. This project was funded by the Austrian Science Fund (FWF; project P19592-B17).

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Correspondence to Luis Cadahía.

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W. Arthofer and L. Cadahía have contributed equally to this work.

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Arthofer, W., Cadahía, L. & Kruckenhauser, L. Ten new microsatellite loci for analysis of genetic diversity in isolated populations of the Alpine land snail Cylindrus obtusus . Conserv Genet 11, 1115–1118 (2010). https://doi.org/10.1007/s10592-009-9893-1

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Keywords

  • Cylindrus obtusus
  • Alpine distribution
  • Isolated populations
  • Microsatellite markers