Conservation Genetics

, Volume 11, Issue 3, pp 1115–1118 | Cite as

Ten new microsatellite loci for analysis of genetic diversity in isolated populations of the Alpine land snail Cylindrus obtusus

  • Wolfgang Arthofer
  • Luis Cadahía
  • Luise Kruckenhauser
Technical Note

Abstract

In this study we characterized 10 polymorphic microsatellite markers for the land snail Cylindrus obtusus, an endemism of the Austrian Alps with a distribution in isolated populations above approximately 1,600 m. The microsatellite loci were analyzed in 44 individuals from two populations. Number of alleles per locus ranged between two and eight. Observed heterozygosity ranged between 0.00 and 1.00, and expected heterozygosity between 0.09 and 0.72. No significant linkage disequilibrium was found between pairs of loci. One of the sampled populations (Dachstein) showed no deviation from Hardy–Weinberg equilibrium and no presence of null alleles, whereas the other one (Schneeberg) did. These diverging results probably reflect differences in population structure rather than characteristics of the microsatellite loci and underline the usefulness of these markers for studying genetic diversity, population structure and differentiation in C. obtusus.

Keywords

Cylindrus obtusus Alpine distribution Isolated populations Microsatellite markers 

Notes

Acknowledgments

We are grateful to Helmut Sattmann who initiated the work with C. obtusus and collected the snails. We thank Barbara Däubl and Simone Latkolik for technical assistance, Christian Stauffer for providing the library enrichment facilities and W. Pinsker and E. Haring for providing useful comments on the manuscript. This project was funded by the Austrian Science Fund (FWF; project P19592-B17).

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Copyright information

© Springer Science+Business Media B.V. 2009

Authors and Affiliations

  • Wolfgang Arthofer
    • 1
  • Luis Cadahía
    • 2
  • Luise Kruckenhauser
    • 2
  1. 1.Institute of Forest Entomology, Forest Pathology and Forest Protection, Department of Forest & Soil SciencesUniversity of Natural Resources and Applied Life Sciences (Boku)ViennaAustria
  2. 2.Lab for Molecular Systematics, 1st Zoological DepartmentMuseum of Natural History ViennaViennaAustria

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