Abstract
A microsatellite-enriched genomic DNA library of Navodon septentrionalis was generated and screened by sequencing. Ten dinucleotide microsatellite loci were characterized by genotyping 24 samples. The observed number of alleles ranged from two to seven with an average of 4.40, while the effective number of alleles ranged from 1.49 to 5.70 with an average of 3.31. The observed and expected heterozygosities ranged from 0.2917 to 0.9167 and from 0.3369 to 0.8422, respectively. No significant linkage disequilibrium between pairs of loci was found, but one loci significantly deviated from Hardy–Weinberg equilibrium after Bonferroni correction. These polymorphic microsatellite loci would be useful for investigating genetic population structure and molecule-assisted breeding for N. septentrionalis.
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Acknowledgments
This study was supported by National Public Platform of Science & Technology Information Resources (2005DKA30470-06), Taishan Scholar Project of Shandong Province and scientific foundation of YSFRI, CAFS (2007-qn-13).
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Xu, GB., Chen, SL. & Tian, YS. New polymorphic microsatellite markers for bluefin leatherjacket (Navodon septentrionalis Gunther, 1877). Conserv Genet 11, 1111–1113 (2010). https://doi.org/10.1007/s10592-009-9891-3
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DOI: https://doi.org/10.1007/s10592-009-9891-3