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Isolation and characterization of 16 microsatellite loci in an endangered fish Ussuri cisco, Coregonus usssruensis

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Abstract

Sixteen polymorphic microsatellite loci were isolated from an enriched genomic library of Ussuri cisco, Coregonus usssruensi. The polymorphism of these loci was tested on a population of 30 individuals captured from Heilongjiang River. The number of observed alleles per locus ranged from 3 to 18. The expected and observed heterozygosity values ranged from 0.126 to 0.919 and 0.133 to 0.833, respectively. Five loci significantly deviated from Hardy–Weinberg equilibrium after Bonferroni correction. No significant linkage disequilibrium was detected. These polymorphic markers will be used to assess population structure in Ussuri cisco.

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Acknowledgments

We acknowledge the financial support of Excellent Young Scientist Foundation of Guangdong Academy of Sciences (No. 2008), and the Foundation of Guangdong Public Lab for Wildlife Conservation & Wise Use (No. 2009).

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Authors and Affiliations

  1. South China Institute of Endangered Animals, No. 105 Xin Gang Xi Road, 510260, Guangzhou, China

    Dongmei Yu & Jianjun Peng

  2. Heilongjiang River Fisheries Research Institute, Chinese Academy of Fisheries Sciences, Harbin, 150070, China

    Bo Ma

  3. School of Life Science, Huazhong Normal University, 430079, Wuhan, China

    Yanhong Sun

Authors
  1. Dongmei Yu
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  2. Bo Ma
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  3. Yanhong Sun
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  4. Jianjun Peng
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Correspondence to Jianjun Peng.

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Yu, D., Ma, B., Sun, Y. et al. Isolation and characterization of 16 microsatellite loci in an endangered fish Ussuri cisco, Coregonus usssruensis . Conserv Genet 11, 1107–1109 (2010). https://doi.org/10.1007/s10592-009-9890-4

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  • DOI: https://doi.org/10.1007/s10592-009-9890-4

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