Abstract
We have developed eight di- and tetranucleotide Lissotriton microsatellite markers. Eight loci were polymorphic in the palmate newt Lissotriton helveticus and six were polymorphic in the smooth newt L. vulgaris. Polymorphism detected in 33 and 37 individuals per species ranged from 3 to 15 alleles. These markers are suitable for the investigation of population structure, genetic variation and taxonomic identification in the two focal species, and may also be of use in other Lissotriton–Triturus species.
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Acknowledgments
Jagroop Pandhal and Mark Sztatecsny kindly tested some loci for polymorphism. We thank Région Bretagne for their financial support for analyses in the Plateforme OUEST Genopole (INRA, Rennes) and Solène Coedel who welcomed us. This study was funded by Région Pays-de-la-Loire, DIREN Pays-de-la-Loire and Angers Loire Métropole.
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Johanet, A., Picard, D., Garner, T.W.J. et al. Characterization of microsatellite loci in two closely related Lissotriton newt species. Conserv Genet 10, 1903–1906 (2009). https://doi.org/10.1007/s10592-009-9850-z
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DOI: https://doi.org/10.1007/s10592-009-9850-z