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Isolation and characterization of ten new polymorphic microsatellite loci in the mud crab, Scylla paramamosain

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Abstract

We developed polymerase chain primers for ten microsatellite loci in the mud crab, Scylla paramamosain. All markers were obtained from a (CA)15 and (CT)15-enrichment DNA library, and characterized in 30 individuals from one wild population. The number of alleles per locus varies between 8 and 18, and the observed and expected heterozygosities ranged from 0.6207 to 0.9333 and from 0.5886 to 0.9243, respectively. These polymorphic loci provide a valuable tool for population genetic analysis and parentage determination in this species.

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Acknowledgments

This work was funded by a grant (2006AA10A406) from the National High Technology Research and Development Program of China (863 Program).

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Correspondence to Gui-Zhong Wang.

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Xu, XJ., Wang, GZ., Wang, KJ. et al. Isolation and characterization of ten new polymorphic microsatellite loci in the mud crab, Scylla paramamosain . Conserv Genet 10, 1877–1878 (2009). https://doi.org/10.1007/s10592-009-9843-y

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  • DOI: https://doi.org/10.1007/s10592-009-9843-y

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