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Isolation and characterization of ten new polymorphic microsatellite loci in the mud crab, Scylla paramamosain

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We developed polymerase chain primers for ten microsatellite loci in the mud crab, Scylla paramamosain. All markers were obtained from a (CA)15 and (CT)15-enrichment DNA library, and characterized in 30 individuals from one wild population. The number of alleles per locus varies between 8 and 18, and the observed and expected heterozygosities ranged from 0.6207 to 0.9333 and from 0.5886 to 0.9243, respectively. These polymorphic loci provide a valuable tool for population genetic analysis and parentage determination in this species.

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  • Clarke KR, Gorley RN (2001) PRIMER v5: user manual/tutorial. PRIMER-E Ltd, Plymouth, p 91

    Google Scholar 

  • Keenan CP (1999) Aquaculture of the mud crab, genus Scylla—past, present and future. In: Keenan CP, Blackshaw A (eds) Mud crab aquaculture and biology. ACIAR Proceedings, China, vol 78, p 9–13

  • Raymond M, Rousset F (1995) GENEPOP: population genetics software for exact tests and ecumenicism. J Hered 86:248–249

    Google Scholar 

  • Zane L, Bargelloni L, Patarnello T (2002) Strategies for microsatellite isolation: a review. Mol Ecol 11:1–16. doi:10.1046/j.0962-1083.2001.01418.x

    Article  CAS  PubMed  Google Scholar 

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This work was funded by a grant (2006AA10A406) from the National High Technology Research and Development Program of China (863 Program).

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Correspondence to Gui-Zhong Wang.

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Xu, XJ., Wang, GZ., Wang, KJ. et al. Isolation and characterization of ten new polymorphic microsatellite loci in the mud crab, Scylla paramamosain . Conserv Genet 10, 1877–1878 (2009).

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