Development of micro satellite markers for a critically endangered species, Ceropegia fantastica from the Western Ghats, India
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Ceropegia fantastica L. (Asclepiadaceae) is a highly endemic and endangered species in the Western Ghats of India. Fourteen microsatellite markers were developed for C. fantastica. Eight microsatellite primers screened had 2–5 alleles per locus and the observed and expected heterozygosity ranged from 0.48 to 0.83 and 0.48 to 0.62, respectively. The primers were also evaluated for their cross amplification against two related species Ceropegia hirsuta and Ceropegia oculata. The microsatellites developed for this species could be used for addressing population genetics of this endemic and critically endangered species.
KeywordsMicrosatellites Ceropegia fantastica Western Ghats Asclepiadaceae Cross amplification Endangered species
This work was funded to RG by the Department of Biotechnology (DBT), Government of India under the species recovery programme for Ceropegia fantastica (BT/7055/BCE/08/441/2006). We thank Prof. S. R. Yadav, Shivaji University, Kolhapur for assistance in collection and identification of the species.
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