Skip to main content

Characterization of 18 microsatellite marker loci in the white-collared lemur (Eulemur cinereiceps)

Conservation Genetics volume 10, pages 1459–1462 (2009)Cite this article

Abstract

The white-collared lemur (Eulemur cinereiceps) is found in southeastern Madagascar. This species is endangered due to the loss and fragmentation of its natural habitat and hunting. Eighteen nuclear microsatellite loci were isolated from genomic DNA derived from an animal from Mahabo Forest in Madagascar. Population genetic parameters were estimated from a population of white-collared lemur sampled at Vevembe Forest 7 years apart to evaluate the potential utility of this marker suite.

This is a preview of subscription content, access via your institution.

Access options

Buy single article

Instant access to the full article PDF.

USD 39.95

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

References

  • Dieringer D, Schlötterer C (2003) Microsatellite analyzer (MSA): a platform independent analysis tool for large microsatellite data sets. Mol Ecol Notes 3:167–169. doi:10.1046/j.1471-8286.2003.00351.x

    Article  CAS  Google Scholar 

  • Goudet J (1995) FSTAT, a computer program to test F-statistics. J Hered 86:85–486

    Google Scholar 

  • Goudet J (2001) FSTAT, a program to estimate and test gene diversities and fixation indices (version 2.9.3), Updated from Goudet (1995)

  • Hillis DM, Mable BK, Larson A, Davis SK, Zimmer EA (1996) Nucleic acids IV: sequencing and cloning. In: Hillis DM, Moritz C, Zimmer EA (eds) Molecular systematics, 2nd edn. Sinauer Associates, Inc., Sunderland, pp 321–381

    Google Scholar 

  • Irwin MT, Johnson SE, Wright PC (2005) The state of lemur conservation in southeastern Madagascar: population and habitat assessment for diurnal and cathemeral lemurs using surveys, satellite imagery and GIS. Oryx 39:204–218. doi:10.1017/S0030605305000451

    Article  Google Scholar 

  • Johnson SE, Wyner Y (2000) Notes on the biogeography of Eulemur albocollaris. Lemur News 5:25–28

    Google Scholar 

  • Johnson SE, Gordon AD, Stumpf RM, Overdorff DJ, Wright PC (2005) Morphological variations in populations of Eulemur albocollaris and E. fulvus rufus. Int J Primatol 26(6):1399–1417. doi:10.1007/s10764-005-8858-6

    Article  Google Scholar 

  • Johnson SE, Lei R, Martin SK, Irwin MT, Louis EE, Jr (2008) Does Eulemur cinereiceps exist? Preliminary evidence from genetics and ground surveys in southeastern Madagascar. Am J Primatol 70(4):372–385

    Article  CAS  PubMed  Google Scholar 

  • Kandpal RP, Kandpal G, Weissman SM (1994) Construction of libraries enriched for sequence repeats and jumping clones, and hybridization selection for region-species markers. Proc Natl Acad Sci USA 91:88–92. doi:10.1073/pnas.91.1.88

    Article  CAS  PubMed  Google Scholar 

  • Longmire JL, Gee GF, Hardekoff CL, Mark GA (1992) Establishing paternity in whooping cranes (Grus americana) by DNA analysis. Auk 109:522–529

    Google Scholar 

  • Marshall TC, Slate J, Kruuk LEB, Pemberton JM (1998) Statistical confidence for likelihood-based paternity inference in natural populations. Mol Ecol 7(5):639–655. doi:10.1046/j.1365-294x.1998.00374.x

    Article  CAS  PubMed  Google Scholar 

  • Mittermeier RA, Ratsimbazafy J, Rylands AB, Williamson L, Oates JF, Mbora D, Ganzhorn JU, Rodríguez-Luna E, Palacios E, Heymann EW, Kierulff MCM, Yongcheng L, Supriatna J, Roos C, Walker S, Aguiar JM (2007) Primates in peril: the world’s 25 most endangered primates 2006–2008. Prim Conserv 22:1–40

    Article  Google Scholar 

  • Moraga-Amador D, Farmerie BA, Brazeau D, Clark G (2001) Tools for developing molecular markers. In: Interdisciplinary center for biotechnology research laboratory manual. The University of Florida Gainesville, Florida, pp 11–65

  • Petter JJ, Petter-Rousseaux A (1979) Classification of the prosimians. In: Doyle GA, Martin RD (eds) The study of prosimian behavior. Academic Press, London, pp 359–409

    Google Scholar 

  • Raymond M, Rousset F (1995) GENEPOP (version 1.2): population genetics software for exact tests and ecuminicisms. J Hered 86:248–249

    Google Scholar 

  • Sambrook J, Fritch EF, Maniatus T (1989) Molecular cloning: a laboratory manual, 2nd edn. Cold Spring Harbor Press, New York

    Google Scholar 

  • Slate J, Marshall TC, Pemberton JM (2000) A retrospective assessment of the accuracy of the paternity inference program CERVUS. Mol Ecol 9(6):801–808. doi:10.1046/j.1365-294x.2000.00930.x

    Article  CAS  PubMed  Google Scholar 

  • Tattersall I (1982) The primates of Madagascar. Columbia University Press, New York

    Google Scholar 

  • Van Oosterhaut C, Hutchinson WF, Wills DPM, Shipley P (2004) MICRO-CHECKER: software for identifying and correcting genotyping errors in microsatellite data. Mol Ecol Notes 4:535–538. doi:10.1111/j.1471-8286.2004.00684.x

    Article  Google Scholar 

Download references

Acknowledgments

We are grateful to CAFF/CORE for permission to conduct this research in Madagascar. The authors wish to acknowledge the generosity of Bill and Berniece Grewcock for their support of student interns. We thank the Theodore F. and Claire M. Hubbard Family Foundation for their support of the Henry Doorly Zoo/Madagascar Biodiversity and Biogeography Project. This project was aided by the support of the staff of the Institute for Conservation of Tropical Environments, Madagascar (ICTE-MICET). Research was also supported by grants from the NSF, Wenner-Gren, IIE Fulbright, the Ahmanson Foundation, and the University of Calgary.

Author information

Authors and Affiliations

  1. Department of Animal Biology, Faculty of Sciences, University of Antananarivo, D.P. 906, Antananarivo, Madagascar

    Hobinjatovo Tokiniaina

  2. Center for Conservation and Research, Henry Doorly Zoo, 3701 South 10th Street, Omaha, NE, 68107, USA

    Carolyn A. Bailey, Gary D. Shore, Edward E. Louis & Rick A. Brenneman

  3. Department of Anthropology, University of Calgary, 2500 University Drive NW, Calgary, AB, Canada, T2N 1N4

    Kira E. Delmore & Steig E. Johnson

Authors
  1. Hobinjatovo Tokiniaina
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Carolyn A. Bailey
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Gary D. Shore
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Kira E. Delmore
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Steig E. Johnson
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Edward E. Louis
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Rick A. Brenneman
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Rick A. Brenneman.

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Tokiniaina, H., Bailey, C.A., Shore, G.D. et al. Characterization of 18 microsatellite marker loci in the white-collared lemur (Eulemur cinereiceps). Conserv Genet 10, 1459–1462 (2009). https://doi.org/10.1007/s10592-008-9760-5

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10592-008-9760-5

Keywords

  • Genetic markers
  • White-collared lemur
  • Eulemur cinereiceps
  • Microsatellites