Characterization of 18 microsatellite marker loci in the white-collared lemur (Eulemur cinereiceps)
The white-collared lemur (Eulemur cinereiceps) is found in southeastern Madagascar. This species is endangered due to the loss and fragmentation of its natural habitat and hunting. Eighteen nuclear microsatellite loci were isolated from genomic DNA derived from an animal from Mahabo Forest in Madagascar. Population genetic parameters were estimated from a population of white-collared lemur sampled at Vevembe Forest 7 years apart to evaluate the potential utility of this marker suite.
KeywordsGenetic markers White-collared lemur Eulemur cinereiceps Microsatellites
We are grateful to CAFF/CORE for permission to conduct this research in Madagascar. The authors wish to acknowledge the generosity of Bill and Berniece Grewcock for their support of student interns. We thank the Theodore F. and Claire M. Hubbard Family Foundation for their support of the Henry Doorly Zoo/Madagascar Biodiversity and Biogeography Project. This project was aided by the support of the staff of the Institute for Conservation of Tropical Environments, Madagascar (ICTE-MICET). Research was also supported by grants from the NSF, Wenner-Gren, IIE Fulbright, the Ahmanson Foundation, and the University of Calgary.
- Goudet J (1995) FSTAT, a computer program to test F-statistics. J Hered 86:85–486Google Scholar
- Goudet J (2001) FSTAT, a program to estimate and test gene diversities and fixation indices (version 2.9.3), Updated from Goudet (1995)Google Scholar
- Hillis DM, Mable BK, Larson A, Davis SK, Zimmer EA (1996) Nucleic acids IV: sequencing and cloning. In: Hillis DM, Moritz C, Zimmer EA (eds) Molecular systematics, 2nd edn. Sinauer Associates, Inc., Sunderland, pp 321–381Google Scholar
- Johnson SE, Wyner Y (2000) Notes on the biogeography of Eulemur albocollaris. Lemur News 5:25–28Google Scholar
- Longmire JL, Gee GF, Hardekoff CL, Mark GA (1992) Establishing paternity in whooping cranes (Grus americana) by DNA analysis. Auk 109:522–529Google Scholar
- Mittermeier RA, Ratsimbazafy J, Rylands AB, Williamson L, Oates JF, Mbora D, Ganzhorn JU, Rodríguez-Luna E, Palacios E, Heymann EW, Kierulff MCM, Yongcheng L, Supriatna J, Roos C, Walker S, Aguiar JM (2007) Primates in peril: the world’s 25 most endangered primates 2006–2008. Prim Conserv 22:1–40CrossRefGoogle Scholar
- Moraga-Amador D, Farmerie BA, Brazeau D, Clark G (2001) Tools for developing molecular markers. In: Interdisciplinary center for biotechnology research laboratory manual. The University of Florida Gainesville, Florida, pp 11–65Google Scholar
- Petter JJ, Petter-Rousseaux A (1979) Classification of the prosimians. In: Doyle GA, Martin RD (eds) The study of prosimian behavior. Academic Press, London, pp 359–409Google Scholar
- Raymond M, Rousset F (1995) GENEPOP (version 1.2): population genetics software for exact tests and ecuminicisms. J Hered 86:248–249Google Scholar
- Sambrook J, Fritch EF, Maniatus T (1989) Molecular cloning: a laboratory manual, 2nd edn. Cold Spring Harbor Press, New YorkGoogle Scholar
- Tattersall I (1982) The primates of Madagascar. Columbia University Press, New YorkGoogle Scholar