Abstract
Eleven novel microsatellite markers were isolated from small abalone, Haliotis diversicolor Reeve. These loci were tested on 22 individuals from two different geographic populations. We identified a total of 162 alleles from the 11 microsatellite loci. All of the loci were highly polymorphic. Polymorphism information content (PIC) is ranging from 0.7276 to 0.9163. Observed and expected heterozygosities ranged from 0.2727 to 1.0000 and from 0.7738 to 0.9429, respectively. Three loci deviated significantly from Hardy–Weinberg equilibrium. No pairs of loci displayed linkage disequilibrium. These polymorphic markers will be used to analyze population structure, genetic diversity and construct a genetic linkage map.
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Acknowledgments
Many thanks to Dr. Geng Jia-ning and Dr. Li Ke-xin for their useful suggestions and assistant Jia Ruo-yu for technical assistance with genotyping analysis. We thank Dr. You Wei-wei and Mr. Wang Bo for care of experimental samples. The project was supported by the National High Technology Research and Development Program of China (2006AA10A407).
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Xin Zhan and Hai-Yan Hu contribute equally to this study.
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Zhan, X., Hu, HY., Ke, CH. et al. Isolation and characterization of eleven microsatellite loci in small abalone, Haliotis diversicolor Reeve. Conserv Genet 10, 1185–1187 (2009). https://doi.org/10.1007/s10592-008-9740-9
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DOI: https://doi.org/10.1007/s10592-008-9740-9