Abstract
The ark shell Scapharca subcrenata is an important fishery resource, but has been suffering from severe population decline due to the deterioration of the coastal environment and over-exploitation. To investigate the genetic variation and structure among populations of S. subcrenata, 14 polymorphic microsatellite loci were developed. The number of alleles per polymorphic locus ranged from 11 to 29 and the observed and expected heterozygosity varied from 0.400 to 0.923 and from 0.705 to 0.965, respectively. These microsatellites will help advance the investigation of the genetic population structure and genetic diversity in this species.
References
Dieringer D, Schlötterer C (2003) Microsatellite analyser (MSA): a platform independent analysis tool for large microsatellite data sets. Mol Ecol Notes 3:167–169. doi:10.1046/j.1471-8286.2003.00351.x
Gardner MG, Cooper SJB, Bull CM, Grant WN (1999) Isolation of microsatellite loci from a social lizard, Egernia stokesii, using a modified enrichment procedure. J Hered 90:301–304. doi:10.1093/jhered/90.2.301
Li Q, Yu H, Yu RH (2006) Genetic variability assessed by microsatellites in cultured populations of the Pacific oyster (Crassostrea gigas) in China. Aquaculture 259:95–102. doi:10.1016/j.aquaculture.2006.05.030
Li XG, Yan BL, Xu GP, Chen JH, Dong ZG, Wu XB et al (2007) Analysis on biochemical genetic variation in three geographical populations of Scapharca subcrenata. Mar Fish 29:207–213 (in Chinese)
Rousset F (2008) GENEPOP’007: a complete re-implementation of the GENEPOP software for Windows and Linux. Mol Ecol Resour 8:103–106. doi:10.1111/j.1471-8286.2007.01931.x
Xu XH, Yan BL, Xu GC, Shi DQ, Zhu ZHQ (2005a) A morphologic, histological study on the ovary of Scapharca subcrenata. J Huaihai Inst Technol 14:66–68 (in Chinese)
Xu XH, Yan BL, Zheng JS, Xu GC, Shi DQ (2005b) Morphological, histological and histochemical studies on the digestive system of Scapharca subcrenata (Lischke). Trans Oceanol Limnol 3:23–30 (in Chinese)
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The study was supported by the grants from National High Technology Research and Development Program (2007AA09Z433), and Chinese Ministry of Education (707041).
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Feng, YW., Li, Q. & Kong, LF. Isolation and characterization of 14 polymorphic microsatellite loci in the ark shell Scapharca subcrenata (Bivalvia: Arcidae). Conserv Genet 10, 1125–1127 (2009). https://doi.org/10.1007/s10592-008-9725-8
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DOI: https://doi.org/10.1007/s10592-008-9725-8