Abstract
We developed eight polymorphic microsatellite markers for the endangered salmonid Parahucho perryi from genomic libraries enriched for (GACA)n and (GATA)n repeat sequences. Emphasis was placed on developing highly polymorphic, perfect repeats that could be scored with confidence. Six tetra- and two di-nucleotide loci were screened in 49 individuals from two populations on Sakhalin Island, Russia. Allelic variation was high with eight to 23 alleles per locus and expected heterozygosity ranging from 0.48 to 0.93. These highly variable markers should prove useful in evaluating inbreeding, gene flow and population structure in Sakhalin taimen throughout its range.
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Acknowledgments
We would like to thank Dr. Sergei Safronov of Sakhalin State University for providing tissue samples from field surveys conducted in Sakhalin in 1997. Kathrin Winkler was supported by a grant from the Austrian Science Foundation (FWF L229–B12).
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Kopun, T., Winkler, K.A. & Weiss, S. Eight new polymorphic microsatellite DNA markers for Sakhalin taimen Parahucho perryi . Conserv Genet 10, 1089–1091 (2009). https://doi.org/10.1007/s10592-008-9716-9
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DOI: https://doi.org/10.1007/s10592-008-9716-9