Abstract
Simple sequence repeat (SSR) markers were developed for Japanese white birch, Betula platyphylla var. japonica, using previously designed primer pairs derived from expressed sequence tags (ESTs). Out of 98 unpublished primer pairs, 35 yielded clear PCR amplification products, 11 of which revealed polymorphism in eight individuals sampled across the species’ range. The number of alleles detected and the expected heterozygosity ranged from 1 to 10 and 0.000 to 0.857, respectively, when these 11 loci were examined in 24 individuals from a single B. platyphylla var. japonica population. In cross-species transferability tests most of the 11 loci were also polymorphic in three other Betula species examined, but not B. maximowicziana. We have now developed a total of 25 polymorphic EST-SSRs for the genus Betula (including 14 we previously developed), which are likely to be highly useful in studies of various aspects of population genetics, including hybridization dynamics, in the genus.
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Acknowledgements
We are grateful to Associate Professor Susumu Goto at The University of Tokyo, who provided plant materials of B. pendula and B. papyrifera collected from the arboretum garden of the University forest in Hokkaido, The University of Tokyo. This work was partly supported by a Grant-in-Aid (Assurance of Safe Use of Genetically Modified Organisms) from the Research Council, Ministry of Agriculture, Forestry and Fisheries of Japan and a grant for Research on Genetic Guidelines for Restoration Programs using Genetic Diversity Information from the Ministry of Environment, Japan.
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Tsuda, Y., Ueno, S., Ranta, J. et al. Development of 11 EST-SSRs for Japanese white birch, Betula platyphylla var. japonica and their transferability to related species. Conserv Genet 10, 1385–1388 (2009). https://doi.org/10.1007/s10592-008-9701-3
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DOI: https://doi.org/10.1007/s10592-008-9701-3