Abstract
Nine microsatellite loci were isolated and characterized for the endangered and tertiary relict perennial herb, Tricyrtis ishiiana. The number of alleles ranged from 2 to 33. The expected (H E) and observed (H O) heterozygosities were 0.207–0.944 and 0.215–0.813, respectively, from 96 individuals on one population. Five loci exhibited significantly fewer heterozygotes than expected under Hardy–Weinberg equilibrium (P < 0.05). These primers amplifying microsatellites in this species may provide a useful tool for population genetics to establish conservation strategy.
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Acknowledgements
We are grateful to Ms Akiko Sakai (Kanagawa Prefecture Natural Environment Conservation Center) and Asuka Noda (Kyoto University) for their cooperation in collection materials or extracting DNA, respectively. This study was supported by grant for Conservation Genetics for Endangered Plants from Kanagawa Prefecture Natural Environment Conservation Center.
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Setoguchi, H., Mitsui, Y., Ikeda, H. et al. Development and characterization of microsatellite loci in the endangered Tricyrtis ishiiana (Convallariaceae), a local endemic plant in Japan. Conserv Genet 10, 705–707 (2009). https://doi.org/10.1007/s10592-008-9620-3
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DOI: https://doi.org/10.1007/s10592-008-9620-3