Conservation Genetics

, Volume 10, Issue 3, pp 693–696 | Cite as

Characterization of 29 tetranucleotide microsatellite loci in black bear (Ursus americanus) for use in forensic and population applications

  • E. P. Meredith
  • J. A. Rodzen
  • J. D. Banks
  • K. C. Jones
Technical Note

Abstract

We developed 29 forensic quality tetranucleotide microsatellite loci for the identification of individual black bear (Ursus americanus). Allele number averaged 5.35 and ranged from 3 to 11. The eight markers selected for forensic use permit the identification and exclusion of individual bear with a probability of identity of approximately 8.1 × 10−8 (approximately one per 12 million).

Keywords

Black bear Microsatellite Ursus americanus Forensics 

References

  1. Butler J (2001) Forensic DNA typing. Academic Press. San Diego, California, USAGoogle Scholar
  2. Hanson DG (1983) Poaching: oriental demand for ungdam and paws decimates California Bears. Audubon 85(4):127–128Google Scholar
  3. Inman K, Rudin N (2002) An introduction to forensic DNA analysis, 2nd edn. CRC Press, Boca Raton, Florida, USAGoogle Scholar
  4. Jones KC, Levine KF, Banks JD (2000) DNA-based genetic markers in black-tailed and mule deer for forensic applications. Calif Fish and Game 86:115–126Google Scholar
  5. Paetkau D, Calvert W, Stirling I, Strobeck C (1995) Microsatellite analysis of population structure in Canadian polar bears. Mol Ecol 4:347–354PubMedCrossRefGoogle Scholar
  6. Raymond M, Rousset F (1995) GENEPOP (version 1.2): population genetics software for exact tests and ecumenicism. J Hered 86:248–249Google Scholar
  7. Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning, a laboratory manual, 2nd edn. Cold Spring Harbor Laboratory Press, New York, USAGoogle Scholar
  8. Updike D, Bernard L (2004) All around California, responsible people can help keep bears wild. In: DFG News. http://www.dfg.ca.gov/news/news04/04069.html Accessed 3 Mar 2008

Copyright information

© Springer Science+Business Media B.V. 2008

Authors and Affiliations

  • E. P. Meredith
    • 1
  • J. A. Rodzen
    • 1
  • J. D. Banks
    • 1
  • K. C. Jones
    • 2
  1. 1.Wildlife Forensic LaboratoryCalifornia Department of Fish & GameRancho CordovaUSA
  2. 2.Genetic Identification Services, Inc.ChatsworthUSA

Personalised recommendations