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Development of 13 EST-SSRs for Cerasus jamasakura and their transferability for Japanese flowering cherries

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Abstract

Simple sequence repeat (SSR) markers were developed for the flowering cherry Cerasus jamasakura (also known as Prunus jamasakura) using 31,995 expressed sequence tags (ESTs) from the NCBI database. Out of 96 of designed primer pairs, 63 showed clear PCR amplification and 13 of these revealed polymorphism in eight individuals sampled across the species’ range. The number of alleles detected and expected heterozygosity ranged from 1 to 8 and 0.000 to 0.833, respectively, when these 13 loci were examined in 23 individuals from a single population. For all except one of the lcoi, polymorphism was also detected in at least four of six other taxa of flowering cherries examined. The results show that the developed EST-SSRs are highly transferable, and that these markers are likely to be useful in studies of the population genetics of flowering cherries.

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Acknowledgments

We are grateful to Madoka Kimura, Miki Shigekawa, Namiko Yoshida, Reiko Mizusawa and Taiyuu Akamine for their help in sampling the plant material. This research was supported by a grant for Research on Genetic Guideline for Restoration Programs using Genetic Diversity Information from the Ministry of Environment, Japan.

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Authors and Affiliations

  1. Tree Genetics Laboratory, Department of Forest Genetics, Forestry and Forest Products Research Institute (FFPRI), Matsunosato 1, Tsukuba, Ibaraki, 305-8687, Japan

    Yoshiaki Tsuda, Saneyoshi Ueno & Yoshihiko Tsumura

  2. Laboratory of Forest Molecular Ecology, Graduate School of Agriculture, Gifu University, Yanagido 1-1, Gifu, Gifu, 501-1193, Japan

    Shuri Kato

  3. Forest Bio-Research Laboratory 2, Forest Bio-Research Centre, Forestry and Forest Products Research Institute (FFPRI), Matsunosato 1, Tsukuba, Ibaraki, 305-8687, Japan

    Toshio Katsuki

  4. Laboratory of Forest Molecular Ecology, Faculty of Applied Biological Sciences, Gifu University, Yanagido 1-1, Gifu, Gifu, 501-1193, Japan

    Yuzuru Mukai

Authors
  1. Yoshiaki Tsuda
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  2. Saneyoshi Ueno
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  3. Shuri Kato
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  4. Toshio Katsuki
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  5. Yuzuru Mukai
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  6. Yoshihiko Tsumura
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Correspondence to Yoshiaki Tsuda.

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Tsuda, Y., Ueno, S., Kato, S. et al. Development of 13 EST-SSRs for Cerasus jamasakura and their transferability for Japanese flowering cherries. Conserv Genet 10, 685–688 (2009). https://doi.org/10.1007/s10592-008-9615-0

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  • DOI: https://doi.org/10.1007/s10592-008-9615-0

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