Abstract
Hog deer (Axis porcinus) is listed as an endangered species in many countries and its taxonomic status remains ambiguous. In this study, we developed nine novel microsatellite markers using enrichment methods. These polymorphic microsatellite loci showed allele numbers of 2–3 with an average of 2.22 in a group of 26 individuals and observed and expected heterozygosity ranging from 0.044 to 0.619 (average 0.397) and 0.194 to 0.632 (average 0.433) respectively. Linkage disequilibrium was not detected. These markers could be used in the genetic study and taxonomic identification of this species.
This is a preview of subscription content, log in via an institution to check access.
References
Ali S, Ansari S, Ehtesham NZ, Azfer MA, Homkar U, Gopal R, Hasnain SE (1998) Analysis of the evolutionarily conserved repeat motifs in the genome of the highly endangered central Indian swamp deer Cervus duvauceli branderi. Gene 223:361–367
Belkhir K, Borsa P, Chikhi L, Raufaste N, Bonhomme F (1996–2004) GENETIX 4.05, logiciel sous Windows TM pour la génétique des populations. Laboratoire Génome, Populations, Interactions, CNRS UMR 5000, Université de Montpellier II, Montpellier, France
Bloor PA, Barker FS, Watts PC, Noyes HA, Kemp SJ (2001) Microsatellite libraries by enrichment. v1.0, September. http://www.liv.ac.uk/~kempsj/genomics.html
Gilbert C, Ropiquet A, Hassanin A (2006) Mitochondrial and nuclear phylogenies of Cervidae (Mammalia, Ruminantia): systematics, morphology, and biogeography. Mol Phylogenet Evol 40:101–117
Lalitha S (2000) Primer Premier 5. Biotech Softw Internet Rep 1:270–272
Pitra C, Fickel J, Meijaard E, Groves C (2004) Evolution and phylogeny of old world deer. Mol Phylogenet Evol 33:880–895
Rousset F (2008) Genepop’007: a complete re-implementation of the genepop software for Windows and Linux. Mol Ecol Notes 8:103–106
Salim DC, Akimoto AA, Carvalho CB, Oliveira SF, Grisolia CK, Moreira JR, Klautau-Guimaraes MN (2007) Genetic variability in maned wolf based on heterologous short-tandem repeat markers from domestic dog. Genet Mol Res 6:248–257
Sambrook JW, Russell D (2001) Molecular cloning: a laboratory manual, 3rd edn. Cold Spring Harbor Laboratory Press, New York
Van Oosterhout C, Hutchinson WF, Wills DPM, Shipley P (2004) Micro-checker: software for identifying and correcting genotyping errors in microsatellite data. Mol Ecol Notes 4:535–538
Wan Q-H, Zhu L, Wu HUA, Fang S-G (2006) Major histocompatibility complex class II variation in the giant panda (Ailuropoda melanoleuca). Mol Ecol 15:2441–2450
Zhang D, Chen S, Chen S, Zhang D, Gao Q (2007) Patterns of genetic variation in Swertia przewalskii, an endangered endemic species of the Qinghai-Tibet Plateau. Biochem Genet 45:33–50
Zimmermann (1780) Geogr. Gesch. Mensch. Vierf. Thiere, 2:131. In: Don E. Wilson, DeeAnn M. Reeder (eds). 2005. Mammal species of the World. A Taxonomic and Geographic Reference (3rd edn), Johns Hopkins University Press. http://www.bucknell.edu/msw3/
Acknowledgement
We would like to thank Beijing Zoo, Shanghai Zoo and Nanning Zoo for providing us faecal samples and especially Chengdu Zoo for their blood samples. This work was supported by a grant from the state forestry administration of China (No. 2005-4-C04).
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Lian, H., Yu, JQ., Ge, YF. et al. Nine novel microsatellite markers for the hog deer (Axis porcinus). Conserv Genet 10, 681–683 (2009). https://doi.org/10.1007/s10592-008-9614-1
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10592-008-9614-1