Abstract
Species of the genus Munidopsis are typically distributed in bathyal and abyssal zones, but the anchialine species Munidopsis polymorpha is an exception. It inhabits a volcanic tube on Lanzarote Island (Canary Islands, NE Atlantic) and is currently listed as endangered due to its highly restricted distribution and degree of endemism. Microsatellite loci were isolated from partial genomic libraries that had been enriched for AC, ACAG, GATA, AAAC and AAG repeat sequences. Eight loci were polymorphic in a sample of 24 individuals. The number of alleles per locus ranged from 2 to 4 with observed and expected heterozygosities ranging from 0.083 to 0.875 and from 0.080 to 0.681, respectively. These markers will be used to evaluate levels of genetic diversity and inbreeding, providing essential information for the development of a management and conservation strategy for this species.
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References
Acevedo I, Bloor P, Cabezas P, Toledo C, Calvo M, Machordom A (2008) Development of tetranucleotide microsatellite markers for the cushion starfish, Asterina gibbosa, and cross-species amplification. Mol Ecol Res (in press)
Bloor P, De Laguna HB, Kemp SJ (2006) Highly polymorphic tetranucleotide microsatellite loci for the eastern Canary Island lizard, Gallotia atlantica. Mol Ecol Notes 6:737–739
Brownstein MJ, Carpten JD, Smith JR (1996) Modulation of non-templated nucleotide addition by Taq DNA polymerase: primer modifications that facilitate genotyping. Biotechniques 20:1004–1010
Frankham R, Ballou JD, Briscoe DA (2002) Introduction to conservation genetics. Cambridge University Press, Cambridge
González-Pérez JA (1995) Catálogo de los Crustáceos Decápodos de las Islas Canarias. Turquesa, Santa Cruz de Tenerife
Holthuis LB (1973) Caridean shrimp found in land-locked salt-water pools at four Indo-West Pacific localities (Sinai Peninsula, Funafuti Atoll, Maui and Hawai islands), with the description of one new genus and four new species. Zool Verh 128:1–48
Iliffe TM (2000) Anchialine cave biology. In: Wilkens H, Culver DC, Humphreys WF (eds) Ecosystems of the world 30 subterranean ecosystems. Elsevier Science, Amsterdam, pp 59–76
Peakall R, Smouse PE (2006) GENALEX 6: genetic analysis in Excel. Population genetic software for teaching and research. Mol Ecol Notes 6:288–295
Raymond M, Rousset F (1995) GENEPOP (version 1.2): population genetics software for exact tests and ecumenicism. J Hered 86:248–249
Refseth UH, Fangan BM, Jakobsen KS (1997) Hybridization capture of microsatellites directly from genomic DNA. Electrophoresis 18:1519–1523
Rice WR (1989) Analyzing tables of statistical tests. Evolution 43:223–225
Rozen S, Skaletsky H (2000) Primer3 on the WWW for general users and for biologist programmers. In: Misener S, Krawetz S (eds) Bioinformatics methods and protocols. Humana Press, Totowa, New Jersey, pp 365–386
Schuelke M (2000) An economic method for the fluorescent labeling of PCR fragments. Nat Biotechnol 18:233–234
Templado J, Calvo M, Garvía A, Luque AA, Maldonado M, Moro L (2004) Guía de invertebrados y peces marinos protegidos por la legislación nacional e internacional. Ministerio de Medio Ambiente, Madrid
Acknowledgments
The authors thank the Gobierno de Canarias and Cabildo de Lanzarote for permission to collect samples of Munidopsis polymorpha. We are also very grateful to the staff at the Complejo de los Jameos del Agua for their assistance during fieldwork. We also thank Ricardo García for assistance in the lab. And finally we thank Fernando Alda for his valuable comments on the manuscript. This study was funded by MEC project CTM2004-01769. PC was supported by a grant from the Consejo Superior de Investigaciones Científicas (CSIC).
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Cabezas, P., Bloor, P., Acevedo, I. et al. Development and characterization of microsatellite markers for the endangered anchialine squat lobster Munidopsis polymorpha . Conserv Genet 10, 673–676 (2009). https://doi.org/10.1007/s10592-008-9611-4
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DOI: https://doi.org/10.1007/s10592-008-9611-4