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Highly polymorphic microsatellite loci for the Parsley frog (Pelodytes punctatus): characterization and testing for cross-species amplification

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Abstract

A microsatellite library was developed using genomic DNA of the Parsley frog, Pelodytes punctatus, an amphibian species which inhabits Mediterranean temporary pond systems. Number of alleles and heterozygosity ranged from 10 to 25 and from 0.66 to 0.90, respectively. Cross-species amplification was successful for 13 of the 15 developed loci for the related species, Pelodytes ibericus. The high levels of polymorphism revealed by these loci will be extremely useful for characterizing the population genetic diversity and structure and to estimate levels of dispersal and gene flow in the species P. punctatus and P. ibericus.

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Acknowledgements

This work was supported by Fundação para a Ciência e Tecnologia (FCT) with support from FEDER (Portugal), through research project PTDC/BIA-BDE/68730/2006, and SFRH/BD/24064/2005 PhD grant (FCT, ESF). We are very grateful to Margarida Machado, João Reis and Luís Cancela da Fonseca from the University of Algarve for their field assistance and their help in collecting samples. We also would like to thank Nuno Ferrand for his helpful comments and suggestions.

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Authors and Affiliations

  1. CCMAR, F.C.M.A—University of Algarve, Gambelas, 8005-139, Faro, Portugal

    Mirjam S. van de Vliet, Onno E. Diekmann & Ester T. A. Serrão

  2. ERENA, Ordenamento e Gestão de Recursos Naturais Lda, Rua Robalo Gouveia, 1-1A, 1900-392, Lisbon, Portugal

    Pedro Beja

  3. CIBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Campus Agrário de Vairão, 4485-66, Vairao, Portugal

    Pedro Beja

Authors
  1. Mirjam S. van de Vliet
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  2. Onno E. Diekmann
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  3. Ester T. A. Serrão
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  4. Pedro Beja
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Correspondence to Mirjam S. van de Vliet.

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van de Vliet, M.S., Diekmann, O.E., Serrão, E.T.A. et al. Highly polymorphic microsatellite loci for the Parsley frog (Pelodytes punctatus): characterization and testing for cross-species amplification. Conserv Genet 10, 665–668 (2009). https://doi.org/10.1007/s10592-008-9609-y

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  • DOI: https://doi.org/10.1007/s10592-008-9609-y

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