Abstract
Microsatellites are important genetic markers both in population genetics and for delimitation of closely related species. However, to develop microsatellites for each target organism is expensive and time consuming. In this study, we have therefore developed 65 new microsatellite primers for the species Draba nivalis and tested cross-species and cross-genus transfer success of these primers for two other genera in the Brassicaceae; Cardamine and Smelowskia. Furthermore, 15 previously developed microsatellites were tested for amplification in these three genera. The microsatellite markers that amplify across these genera may be useful for other genera in the Brassicaceae as well.
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Acknowledgments
Thanks to LG Kvernstuen for extracting DNA; the Rieseberg lab at Indiana University, especially M. Moody for advice and discussions; M.H. Jørgensen for providing fragment lengths for Cardamine; and M. Stoutemyer for providing two primers. This work was funded by grant 146515/420 from the Research Council of Norway to C. Brochmann, and grants from the Fulbright Foundation and Kristine Bonnevie Scholarship to I. Skrede.
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Skrede, I., Carlsen, T., Rieseberg, L.H. et al. Microsatellites for three distantly related genera in the Brassicaceae. Conserv Genet 10, 643–648 (2009). https://doi.org/10.1007/s10592-008-9598-x
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DOI: https://doi.org/10.1007/s10592-008-9598-x