Abstract
We isolated nine new dinucleotide microsatellites for the deep-sea fish Hoplostethus atlanticus. These loci are generally highly polymorphic, with allele number ranging from 3 to 31 and observed heterozygosity ranging from 0.067 to 0.933. In conjuction with previously published loci, these markers will be useful for fine-scale analysis of population structure in this commercially important and threatened species.
References
Amos W, Hoffmann JI, Frodsham A, Zhang L, Best S, Hill AVS (2007) Automated binning of microsatellite alleles: problems and solutions. Mol Ecol Notes 7:10–14
Fischer D, Bachmann K (1998) Microsatellite enrichment in organisms with large genomes (Allium cepa L.). Biotechniques 24:796–802
Francis RICC, Clark MR (2005) Sustainability issues for orange roughy fisheries. Bull Mar Sci 79:337–351
Goudet J (1995) FSTAT (Version 1.2): a computer program to calculate F-statistics. J Hered 86:485–486
Hall TA (1999) BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp Ser 41:95–98
Oke CS, Crozier YC, Crozier RH, Ward RD (1999) Microsatellites from a teleost, orange roughy (Hoplostethus atlanticus), and their potential for determining population structure. Mol Ecol 8:2141–2152
Raymond M, Rousset F (1995) GENEPOP (Version 1.2): population genetics software for exact tests and ecumenicism. J Hered 86:248–249
Roberts C (2002) Deep impact: the rising toll of fishing in the deep sea. TREE 17:242–245
Rozen S, Skaletsky H (2000) Primer3 on the WWW for general users and for biologist programmers. In: Krawetz S, Misener S (eds) Bioinformatics methods and protocols: methods in molecular biology. Humana Press, Totowa, NJ, pp 365–386
Van Oosterhout C, Hutchinson WF, Wills DPM, Shipley P (2004) MICRO-CHECKER: software for identifying and correcting genotyping errors in microsatellite data. Mol Ecol Notes 4:535–538
Acknowledgements
We thank Natural Environment Research Council for financial support and Marine Girard and Pascal Lorance for providing samples from the Hebrides and Bay of Biscay fisheries. This work forms part of the ECOMAR project www.oceanlab.abdn.ac.uk/ecomar/.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
White, T.A., Stamford, J. & Hoelzel, A.R. Nine new microsatellite loci for the orange roughy (Hoplostethus atlanticus). Conserv Genet 10, 601–603 (2009). https://doi.org/10.1007/s10592-008-9587-0
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10592-008-9587-0