Abstract
We used the enriched genomic library method to isolate and characterize dinucleotide microsatellite loci in the least horseshoe bat, Rhinolophus pusillus. Seventeen loci were obtained and tested on 31 individuals sampled from Guangxi Province in southern China. Thirteen of these markers were polymorphic with expected heterozygosity ranging from 0.821 to 0.909. A total of 164 alleles were detected and the number of alleles per locus ranged from 9 to 16 (mean 12.6). These polymorphic markers will be used to assess population structure in R. pusillus. In addition, successful cross-amplification in five congeneric bat species suggests most of these markers will also be useful for studying related species.
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Acknowledgments
We are grateful to Jinshuo Zhang, Libiao Zhang, Guangjian Zhu, and Li Wei for assistance with sample collection. This work was funded by a grant under the Key Construction Program of the National “985” Project and Shanghai Priority Academic Discipline awarded to SZ. SJR was supported by a Royal Society Research Fellowship.
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Hua, P., Guo, T., Liu, W. et al. Isolation and characterization of 13 microsatellite loci in Rhinolophus pusillus (least horseshoe bat) with cross-amplification in five related species. Conserv Genet 10, 597–600 (2009). https://doi.org/10.1007/s10592-008-9586-1
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DOI: https://doi.org/10.1007/s10592-008-9586-1