Skip to main content
SpringerLink
Log in

Isolation and characterisation of di and tri nucleotide microsatellite loci in Rosmarinus officinalis (Lamiaceae), using enriched genomic libraries

  • Technical Note
  • Published:
Conservation Genetics Aims and scope Submit manuscript

Abstract

An enrichment protocol was used to isolate and characterise microsatellite loci in Rosmarinus officinalis, a Mediterranean chamephyte. Twelve microsatellite loci were characterised and amplified a total of 117 alleles in a sample of 30 individuals from one population, with an average of 9.75 alleles per locus. Observed heterozygosities ranged from 0.333 to 0.900. Cross-species transferability was also assayed in the two other species of the genus. The cumulated probabilities of exclusion for paternity and parentage of the 12 loci were of 0.999971 and 1, respectively, supporting the usefulness of these microsatellite loci for parentage analyses. Nine out of 12 microsatellite loci amplified in the two species and were polymorphic detecting a total of 49 and 45 in R. eriocalyx and R. tomentosus, respectively. Twenty-two alleles were exclusive of R. eriocalyx and 12 of R. tomentosus, additionally, three alleles were shared between these two species but were otherwise absent in the analysed individuals of R. officinalis. In total, this set of markers amplified 154 different microsatellite alleles, supporting their usefulness to conduct population genetic, reproductive biology and hybridisation studies in Rosmarinus.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

References

  • Bozin B, Mlmica-Dukic N, Samojlik I, Jovin E (2007) Antimicrobial and antioxidant properties of rosemary and sage (Rosmarinus officinalis L. and Salvia officinalis L., lamiaceae) essential oils. J Agr food chem 55:7879–7885

    Article  CAS  Google Scholar 

  • Doyle JJ, Doyle JL (1990) Isolation of plant DNA from fresh tissue. Focus 12:13–15

    Google Scholar 

  • Garbarino JA, Troncoso N, Delpiano P, Carvajal L, Russo A (2006) Antioxidant activity analysis for the selection of Rosmarinus officinalis L. Nat Prod Com 1:1123–1128

    CAS  Google Scholar 

  • Gerber S, Mariette S, Streiff R, Bodénès C, Kremer A (2000) Comparison of microsatellites and amplified fragment length polymorphism markers for parentage analysis. Mol Ecol 9:1037–1048

    Article  PubMed  CAS  Google Scholar 

  • Hernández-Bermejo E, Prados J, Díaz A, Herrera-Molina F (2004) Rosmarinus tomentosus Hub.-Mor. et Maire. In: Bañares-Baudet A, Blanca G, Güemes-Heras J, Moreno-Saiz JC, Ortiz S (eds) Atlas y Libro Rojo de la flora vascular Amenazada de España. Ministerio de Medio Ambiente, Madrid, España, pp 830–831

    Google Scholar 

  • Lunt DH, Hutchinson F, Carvalho GR (1999) An efficient method for PCR-based isolation of microsatellite arrays (PIMA). Mol Ecol 8:891–894

    Article  CAS  Google Scholar 

  • Rosselló JA, Cosín R, Boscaiu M, Vicente O, Martínez I, Soriano P (2006) Intragenomic diversity and phylogenetic systematics of wild rosemaries (Rosmarinus officinalis L. s.l., Lamiaceae) assessed by nuclear ribosomal DNA sequences (ITS). Pl Syst Evol 262:1–12

    Article  CAS  Google Scholar 

  • Rosúa JL (1985) El complejo Rosmarinus eriocalyx-tomentosus en la Península Ibérica. An Jard Bot Madrid 37:587–595

    Google Scholar 

  • Rousset F (2008) GENEPOP’007: a complete re-implementation of the GENEPOP software for Windows and Linux. Mol Ecol Res 8:103–106

    Article  Google Scholar 

  • Rozen S, Skaletsky HJ (2000) Primer3 on the WWW for general users and for biologist programmers. In: Krawetz S, Misener S (eds) Bioinformatics methods and protocols: methods in molecular biology. Humana Press, Totowa, NJ, pp 365–386

    Google Scholar 

Download references

Acknowledgements

We thank Dr. M. Palop-Esteban for her help during sampling and Prof. J.F. Mota (University of Almería, Spain) for providing samples of R. eriocalyx. This study has been supported by a Project grant “Ayudas para la realización de proyectos de I+D+i para equipos de investigación emergentes o de reciente creación (Ref. GV/2007/259)” from the Conselleria d’Empresa, Universitat i Ciencia, Generalitat Valenciana. J. G. Segarra-Moragues was supported by a Itinerario Integrado de Inserción Profesional (I3P) postdoctoral grant from the Spanish Consejo Superior de Investigaciones Científicas (CSIC) and G. Gleiser was supported by a FPU PhD grant from the Spanish Ministerio de Educación y Ciencia.

Author information

Authors and Affiliations

  1. Centro de Investigaciones sobre Desertificación (CIDE-CSIC-UV-GV), Camí de la Marjal s/n, 46470, Albal, Valencia, Spain

    J. G. Segarra-Moragues & G. Gleiser

Authors
  1. J. G. Segarra-Moragues
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. G. Gleiser
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to J. G. Segarra-Moragues.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Segarra-Moragues, J.G., Gleiser, G. Isolation and characterisation of di and tri nucleotide microsatellite loci in Rosmarinus officinalis (Lamiaceae), using enriched genomic libraries. Conserv Genet 10, 571–575 (2009). https://doi.org/10.1007/s10592-008-9572-7

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10592-008-9572-7

Keywords

Search

Navigation