Abstract
This paper describes the characterization of the population structure of an ex situ population of Phalaenopsis gigantea, an endangered epiphytic orchid endemic to Sabah, Malaysia, using 30 polymorphic microsatellite loci. The objective of this investigation was the estimation of the genetic distance between accessions within an ex situ population on the basis of which a conservation strategy aimed at selective breeding and restoration was developed.
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References
Brachet S, Jubier MF, Richard M, Jung-Muller B, Frascaria-Lacoste N (1999) Rapid isolation of microsatellite loci using 5′ anchored PCR in the common ash Fraxinus excelsior. Mol Ecol 8:157–168
Dellaporta SL, Hicks JB (1983) A plant DNA minipreparation: version II. Plant Mol Biol Rep 1(14):19–21
Fisher PJ, Gardener RC, Richardson TE (1996) Single locus microsatellites isolated using 5′-anchored PCR. Nucleic Acids Res 24:4369–4437
Nei M (1978) Estimation of average heterozygosity and genetic distance from a small number of individuals. Genetics 89:583–590
Pinheiro F, Santos MO, Barros F, Meyer D, Salatino A, Souza AP, Cozzolino S (2008) Isolation and characterization of microsatellite loci in the Brazilian orchid Epidendrum fulgens. Conserv Genet. doi: 10.1007/s10592-008-9514-4
Rozen S, Skaletsky HJ (2000) PRIMER3 on the WWW for general users and for biologist programmers. In: Krawetz S, Misener S (eds) Bioinformatics methods and protocols: methods in molecular biology. Humana Press, Totowa, New Jersey, pp 365–386
Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning: a laboratory manual, 2nd edn. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York
Schlotterer C (1998) Microsatellites. In: Hoelzel A (ed) Molecular genetic analysis of populations: a practical approach. Oxford University Press, Oxford, pp 237–261
Taberlet P, Gielly L, Pautou G, Bouvet J (1991) Universal primers for amplification of three non-coding regions of chloroplast DNA. Plant Mol Biol 17:1105–1109
Tamura K, Dudley J, Nei M, Kumar S (2007) MEGA4: molecular evolutionary genetics analysis (MEGA) software version 4.0. Mol Biol Evol 24:1596–1599. (Publication PDF at http://www.kumarlab.net/publications). Accessed 25 Oct 2007
Weir BS (1979) Inferences about linkage disequilibrium. Biometrics 35:235–254
Yeh FC, Yang RC, Boyle T (1999) POPGENE. Microsoft windows based freeware for population genetic analysis. Release 1.31. University of Alberta, Edmonton
Acknowledgements
The authors thank the Ministry of Science, Technology and Innovation of the Government of Malaysia for having fully funded this research through the IRPA grant 01-02-0052-EA0052. The specimens provided for this study were obtained with the kind permission of the Sabah Parks Authority of the Government of Malaysia.
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Rodrigues, K.F., Kumar, S.V. Isolation and characterization of microsatellite loci in Phalaenopsis gigantea . Conserv Genet 10, 559–562 (2009). https://doi.org/10.1007/s10592-008-9569-2
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DOI: https://doi.org/10.1007/s10592-008-9569-2