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Isolation and characterization of polymorphic microsatellite DNA markers in the rock bream (Oplegnathus fasciatus)

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Abstract

From a (GT)13-enriched genomic library of Oplegnathus fasciatus, 14 polymorphic microsatellite were isolated and characterized in a test population with alleles ranging from two to nine, the observed and expected heterozygosities from 0.0000 to 1.0000, and from 0.1726 to 0.8507, respectively. Five loci deviated from the Hardy–Weinberg equilibrium, and linkage disequilibrium between two loci was significant. Two loci were also polymorphic in Pagrosomus major assessed for cross-species amplification. These polymorphic microsatellites will be useful for genetic diversity analysis and molecule-assisted breeding for O. fasciatus.

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Acknowledgement

This study was supported by State 863 High-technology R&D project of China (2006AA10A403), Taishan Scholar Project of Shandong Province, China and Scientific foundation of YSFRI, CAFS (2007-qn-13).

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Authors and Affiliations

  1. Key Laboratory for Sustainable Utilization of Marine Fisheries Resource, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Nanjing Road 106, Qingdao, 266071, China

    Tianjun Xu, Changwei Shao, Xiaolin Liao, Xiangshan Ji & Songlin Chen

  2. College of Aqua-life Science and Technology, Shanghai Fisheries University, Shanghai, 200090, China

    Tianjun Xu

Authors
  1. Tianjun Xu
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  2. Changwei Shao
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  3. Xiaolin Liao
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  4. Xiangshan Ji
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  5. Songlin Chen
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Corresponding author

Correspondence to Songlin Chen.

Additional information

Changwei Shao contributed equally.

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Xu, T., Shao, C., Liao, X. et al. Isolation and characterization of polymorphic microsatellite DNA markers in the rock bream (Oplegnathus fasciatus). Conserv Genet 10, 527–529 (2009). https://doi.org/10.1007/s10592-008-9557-6

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  • DOI: https://doi.org/10.1007/s10592-008-9557-6

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