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Isolation and characterization of 12 novel microsatellite loci for the red panda (Ailurus fulgens)

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Abstract

The information on dispersal patterns and mating systems of red pandas is quite important for the understanding of the genetic diversity and divergence of this species. And microsatellite marker is an ideal tool to analyze dispersal patterns and mating systems. Thus, we describe in this paper the isolation and characterization of 12 microsatellite loci in the red panda from genomic DNA-enriched libraries. These loci were highly polymorphic with numbers of alleles per locus in 24 individuals ranging from 2 to 14, observed heterozygosity from 0.143 to 0.864 and expected heterozygosity from 0.297 to 0.872. All loci except for RP6 locus followed Hardy–Weinberg expectations. No significant linkage association was found among all these loci. The 12 novel polymorphic microsatellite loci will be of use in studying dispersal patterns and mating systems of red pandas.

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Acknowledgements

This study was supported by projects of the National Basic Research Program of China (973 Program, 2007CB411600), National Natural Science Foundation of China (No.30620130432 and 30770399) and CAS 100 Talent Programme.

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Correspondence to Fu-Wen Wei.

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Wu, H., Zhan, XJ., Guo, Y. et al. Isolation and characterization of 12 novel microsatellite loci for the red panda (Ailurus fulgens). Conserv Genet 10, 523–525 (2009). https://doi.org/10.1007/s10592-008-9556-7

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  • DOI: https://doi.org/10.1007/s10592-008-9556-7

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