Abstract
Fourteen polymorphic microsatellite loci were isolated from the golden pheasant (Chrysolophus pictus). The average allele number of these microsatellites was 11.14 per locus, ranging from two to seventeen in a group of 31 individuals. The mean observed and expected heterozygosities were 0.763 and 0.722, respectively. The average polymorphic information content value, cumulative discrimination power, overall probability of exclusion with both unknown parents or with only unknown sire for these markers were 0.741, 1.0, 0.9999, and 1.0, respectively. This set of microsatellite markers would provide useful tools for conservation genetic studies of the golden pheasant.
References
Delacour J (1977) The pheasants of the world. Spur Publications, Hants, England
Fischer D, Bachmann K (1998) Microsatellite enrichment in organisms with large genomes. Allium cepa L Biotechniques 24:796–802
Genlous S, Bjǒrn S (2003) Microsatellite variability and heterozygote deficiency in the arctic-alpine Alaskan wheatgrass (Elymus alaskanus) complex. Genome 46:729–737
Hamilton MB, Pincus EL, Di Fiore A, Fleischer RC (1999) Universal linker and ligation procedures for construction of genomic DNA libraries enriched for microsatellites. Biotechniques 27:500–507
Kloosterman AD, Budowle B, Daselaar P (1993) PCR-amplification and detection of the human DIS80 VNTR locus. Amplification conditions, population genetics and application in forensic analysis. Int J Legal Med 105:257–264
Li LX, Zhang HJ (2006) Present situation of study on chrysolophus pictus. Sichuan J Zool 25:906–909
Marshall TC, Slate J, Kruuk LEB, Pemberton JM (1998) Statistical confidence for likelihood-based paternity inference in natural populations. Mol Ecol 7:639–655
Sambrook J, Russell DH (2001) Molecular cloning: a laboratory manual. 2nd edn CSHL Press, New York
Van Oosterhout C, Hutchinson WF, Wills DPM, Shipley P (2004) Micro-checker: software for identifying and correcting genotyping errors in microsatellite data. Mol Ecol Notes 4:535–538
Zheng GM (1996) China species red list-bird. Higher Education Press, Beijing, P.R. China
Acknowledgments
This work was supported by grants from the National Basic Research Program of China (973 program) (No.2007CB411600).
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
He, K., Zhang, P., Fang, SG. et al. Development and characterization of 14 novel microsatellite markers from the golden pheasant (Chrysolophus pictus). Conserv Genet 10, 511–513 (2009). https://doi.org/10.1007/s10592-008-9552-y
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10592-008-9552-y