Abstract
The flagfish (Jordanella floridae) is commonly used in studies of wetlands ecology. Here we describe the isolation of ten microsatellite loci, six of which were polymorphic. The observed number of alleles ranged from 4 to 24 and observed heterozygosities ranged from 0.59 to 0.81 for the polymorphic loci. The isolation of these markers will enable estimations of genetic diversity in natural populations.
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Acknowledgements
We thank the University of Florida BEECS Genetic Analysis Laboratory for their logistical support, and in particular, JA Ernst, MS Ascunce, and the ICBR DNA Sequencing Core. We also thank L Bonachea, A Chin, RE Hale, and A Jadidian. Funding was provided by NSF Grant #0135412 (CSt.M) and an NSF Graduate Research Fellow (HK).
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Klug, H., St. Mary, C.M. & Clark, A. Isolation and characterization of microsatellite DNA markers for the flagfish Jordanella floridae . Conserv Genet 9, 1677–1678 (2008). https://doi.org/10.1007/s10592-008-9522-4
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DOI: https://doi.org/10.1007/s10592-008-9522-4