Abstract
Diademed sifaka (Propithecus diadema) distributed throughout Madagascar’s eastern rain forests from the Mangoro and Onive Rivers north to the Mananara River. Twenty-one polymorphic nuclear microsatellite loci were isolated from genomic DNA derived from a P. diadema, from Mantadia National Park. Population genetic parameters were estimated on 10 individuals each, sampled from Mantadia National Park and Maromizaha Unprotected Forest. Allele sizes ranged from 4 to 14 and observed heterozygosities ranged from 0.200 to 0.900 per locus. This marker suite will be informative in further population genetic studies.
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Acknowledgements
The authors wish to acknowledge the generosity of Bill and Berniece Grewcock for their support of student interns. This research was supported by grants from the Ahmanson Foundation, which have provided the laboratory with four ABI automated DNA sequencers. We graciously thank the Theodore F. and Claire M. Hubbard Family Foundation for their support of the Henry Doorly Zoo’s Madagascar Biodiversity and Biogeography Project. This project would not have been possible without staff support of the Institute for Conservation of Tropical Environments, Madagascar (ICTE-MICET), the Association Nationale pour la Gestion des Aires Protégées (ANGAP) and the Ministère des Eaux et Foret, Madagascar
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Ramarokoto, R., Lei, R., Vincent, J. et al. Characterization of twenty-one microsatellites developed from Propithecus diadema . Conserv Genet 9, 1377–1380 (2008). https://doi.org/10.1007/s10592-007-9500-2
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DOI: https://doi.org/10.1007/s10592-007-9500-2